Glycoside hydrolase family 47

Glycosyl hydrolase family 47
Identifiers
Symbol	Glyco_hydro_47
Pfam	PF01532
Pfam clan	CL0059
SCOP	1dl2
SUPERFAMILY	1dl2
CAZy	GH47
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, glycoside hydrolase family 47 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.^[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,^[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.^[5]

Glycoside hydrolase family 47 CAZY GH_47 comprises enzymes with only one known activity; alpha-mannosidase (EC 3.2.1.113).

Alpha-mannosidase is involved in the maturation of Asn-linked oligosaccharides.^[6] The enzyme hydrolyses terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide man(9)(glcnac)(2) in a calcium-dependent manner. The mannose residues are trimmed away to produce, first, man(8)glcnac(2), then a man(5)(glcnac)(2) structure.

References

1. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
2. Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
3. Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
4. Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
5. CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
6. Neame PJ, Lal A, Schutzbach JS, Forsee WT, Moremen KW (1994). "Isolation and expression of murine and rabbit cDNAs encoding an alpha 1,2-mannosidase involved in the processing of asparagine-linked oligosaccharides". J. Biol. Chem. 269 (13): 9872–9881. PMID 8144580.