HSPA4

Heat shock 70kDa protein 4
Identifiers
Symbols	HSPA4 ; APG-2; HEL-S-5a; HS24/P52; HSPH2; RY; hsp70; hsp70RY
External IDs	OMIM: 601113 MGI: 1342292 HomoloGene: 1624 GeneCards: HSPA4 Gene
Gene ontology Molecular function • protein binding • ATP binding Cellular component • cytosol • extracellular exosome Biological process • response to unfolded protein • protein import into mitochondrial outer membrane • chaperone-mediated protein complex assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	3308	15525
Ensembl	ENSG00000170606	ENSMUSG00000020361
UniProt	P34932	Q61316
RefSeq (mRNA)	NM_002154	NM_008300
RefSeq (protein)	NP_002145	NP_032326
Location (UCSC)	Chr 5: 133.05 – 133.11 Mb	Chr 11: 53.26 – 53.3 Mb
PubMed search

Heat shock 70 kDa protein 4 is a protein that in humans is encoded by the HSPA4 gene.^[1][2]

The protein encoded by this gene was originally suggested to be a member of the heat shock protein 70 family.^[1] However it is now known that human HSPA4 is an equivalent to mouse the Apg-2 protein and is a member of the Hsp110 family.^[3]

Interactions

HSPA4 has been shown to interact with:

• APAF1^[4]
• DNAJB1,^[5]
• HDAC1,^[6]
• HSF1,^[7][8]
• HSPBP1,^[5]
• Histone deacetylase 2,^[6]
• NAD(P)H dehydrogenase (quinone 1),^[9]
• STUB1,^[10] and
• TTC1.^[5]

References

1. Fathallah DM, Cherif D, Dellagi K, Arnaout MA (Jul 1993). "Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5". Journal of Immunology 151 (2): 810–3. PMID 8335910.
2. "Entrez Gene: HSPA4 heat shock 70kDa protein 4".
3. Kaneko Y, Kimura T, Kishishita M, Noda Y, Fujita J (Apr 1997). "Cloning of apg-2 encoding a novel member of heat shock protein 110 family". Gene 189 (1): 19–24. doi:10.1016/S0378-1119(96)00807-4. PMID 9161406.
4. Saleh A, Srinivasula SM, Balkir L, Robbins PD, Alnemri ES (Aug 2000). "Negative regulation of the Apaf-1 apoptosome by Hsp70". Nature Cell Biology 2 (8): 476–83. doi:10.1038/35019510. PMID 10934467.
5. Oh WK, Song J (Aug 2003). "Cooperative interaction of Hsp40 and TPR1 with Hsp70 reverses Hsp70-HspBp1 complex formation". Molecules and Cells 16 (1): 84–91. PMID 14503850.
6. Johnson CA, White DA, Lavender JS, O'Neill LP, Turner BM (Mar 2002). "Human class I histone deacetylase complexes show enhanced catalytic activity in the presence of ATP and co-immunoprecipitate with the ATP-dependent chaperone protein Hsp70". The Journal of Biological Chemistry 277 (11): 9590–7. doi:10.1074/jbc.M107942200. PMID 11777905.
7. Nair SC, Toran EJ, Rimerman RA, Hjermstad S, Smithgall TE, Smith DF (Dec 1996). "A pathway of multi-chaperone interactions common to diverse regulatory proteins: estrogen receptor, Fes tyrosine kinase, heat shock transcription factor Hsf1, and the aryl hydrocarbon receptor". Cell Stress & Chaperones 1 (4): 237–50. doi:10.1379/1466-1268(1996)001<0237:apomci>2.3.co;2. PMC 376461. PMID 9222609.
8. Abravaya K, Myers MP, Murphy SP, Morimoto RI (Jul 1992). "The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression". Genes & Development 6 (7): 1153–64. doi:10.1101/gad.6.7.1153. PMID 1628823.
9. Anwar A, Siegel D, Kepa JK, Ross D (Apr 2002). "Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1". The Journal of Biological Chemistry 277 (16): 14060–7. doi:10.1074/jbc.M111576200. PMID 11821413.
10. Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C (Jun 1999). "Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions". Molecular and Cellular Biology 19 (6): 4535–45. doi:10.1128/mcb.19.6.4535. PMC 104411. PMID 10330192.

Further reading

• Abravaya K, Myers MP, Murphy SP, Morimoto RI (Jul 1992). "The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression". Genes & Development 6 (7): 1153–64. doi:10.1101/gad.6.7.1153. PMID 1628823.
• Liao J, Lowthert LA, Ghori N, Omary MB (Jan 1995). "The 70-kDa heat shock proteins associate with glandular intermediate filaments in an ATP-dependent manner". The Journal of Biological Chemistry 270 (2): 915–22. doi:10.1074/jbc.270.2.915. PMID 7529764.
• Furlini G, Vignoli M, Re MC, Gibellini D, Ramazzotti E, Zauli G, La Placa M (Jan 1994). "Human immunodeficiency virus type 1 interaction with the membrane of CD4+ cells induces the synthesis and nuclear translocation of 70K heat shock protein". The Journal of General Virology 75 (1): 193–9. doi:10.1099/0022-1317-75-1-193. PMID 7906708.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Schulte AM, Fischer S, Sachse GE, Häfner S, Stelck S, Gassen HG (Mar 1997). "Identification and characterization of a novel hsc70-like gene in the human lung tumor cell line HS24". DNA and Cell Biology 16 (3): 257–68. doi:10.1089/dna.1997.16.257. PMID 9115634.
• Kaneko Y, Kimura T, Kishishita M, Noda Y, Fujita J (Apr 1997). "Cloning of apg-2 encoding a novel member of heat shock protein 110 family". Gene 189 (1): 19–24. doi:10.1016/S0378-1119(96)00807-4. PMID 9161406.
• Bruner KL, Derfoul A, Robertson NM, Guerriero G, Fernandes-Alnemri T, Alnemri ES, Litwack G (1998). "The unliganded mineralocorticoid receptor is associated with heat shock proteins 70 and 90 and the immunophilin FKBP-52". Receptors & Signal Transduction 7 (2): 85–98. PMID 9392437.
• Satyal SH, Chen D, Fox SG, Kramer JM, Morimoto RI (Jul 1998). "Negative regulation of the heat shock transcriptional response by HSBP1". Genes & Development 12 (13): 1962–74. doi:10.1101/gad.12.13.1962. PMC 316975. PMID 9649501.
• Naylor DJ, Stines AP, Hoogenraad NJ, Høj PB (Aug 1998). "Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the Co-chaperones of specific Hsp70 members". The Journal of Biological Chemistry 273 (33): 21169–77. doi:10.1074/jbc.273.33.21169. PMID 9694873.
• Melville MW, Tan SL, Wambach M, Song J, Morimoto RI, Katze MG (Feb 1999). "The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity". The Journal of Biological Chemistry 274 (6): 3797–803. doi:10.1074/jbc.274.6.3797. PMID 9920933.
• Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C (Jun 1999). "Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions". Molecular and Cellular Biology 19 (6): 4535–45. doi:10.1128/mcb.19.6.4535. PMC 104411. PMID 10330192.
• Liu H, Vuyyuru VB, Pham CD, Yang Y, Singh B (Jun 1999). "Evidence of an interaction between Mos and Hsp70: a role of the Mos residue serine 3 in mediating Hsp70 association". Oncogene 18 (23): 3461–70. doi:10.1038/sj.onc.1202699. PMID 10376524.
• Nonoguchi K, Itoh K, Xue JH, Tokuchi H, Nishiyama H, Kaneko Y, Tatsumi K, Okuno H, Tomiwa K, Fujita J (Sep 1999). "Cloning of human cDNAs for Apg-1 and Apg-2, members of the Hsp110 family, and chromosomal assignment of their genes". Gene 237 (1): 21–8. doi:10.1016/S0378-1119(99)00325-X. PMID 10524232.
• O'Keeffe B, Fong Y, Chen D, Zhou S, Zhou Q (Jan 2000). "Requirement for a kinase-specific chaperone pathway in the production of a Cdk9/cyclin T1 heterodimer responsible for P-TEFb-mediated tat stimulation of HIV-1 transcription". The Journal of Biological Chemistry 275 (1): 279–87. doi:10.1074/jbc.275.1.279. PMID 10617616.
• Agostini I, Popov S, Li J, Dubrovsky L, Hao T, Bukrinsky M (Sep 2000). "Heat-shock protein 70 can replace viral protein R of HIV-1 during nuclear import of the viral preintegration complex". Experimental Cell Research 259 (2): 398–403. doi:10.1006/excr.2000.4992. PMID 10964507.
• Kim M, Jiang LH, Wilson HL, North RA, Surprenant A (Nov 2001). "Proteomic and functional evidence for a P2X7 receptor signalling complex". The EMBO Journal 20 (22): 6347–58. doi:10.1093/emboj/20.22.6347. PMC 125721. PMID 11707406.
• Johnson CA, White DA, Lavender JS, O'Neill LP, Turner BM (Mar 2002). "Human class I histone deacetylase complexes show enhanced catalytic activity in the presence of ATP and co-immunoprecipitate with the ATP-dependent chaperone protein Hsp70". The Journal of Biological Chemistry 277 (11): 9590–7. doi:10.1074/jbc.M107942200. PMID 11777905.
• Gurer C, Cimarelli A, Luban J (May 2002). "Specific incorporation of heat shock protein 70 family members into primate lentiviral virions". Journal of Virology 76 (9): 4666–70. doi:10.1128/JVI.76.9.4666-4670.2002. PMC 155079. PMID 11932435.
• Freeman BC, Yamamoto KR (Jun 2002). "Disassembly of transcriptional regulatory complexes by molecular chaperones". Science 296 (5576): 2232–5. doi:10.1126/science.1073051. PMID 12077419.

External links

• HSPA4 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)