UBE2M

Ubiquitin-conjugating enzyme E2M
PDB rendering based on 1y8x.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1TT5​, 1Y8X​, 2NVU​, 3TDU​, 3TDZ​, 4GAO​, 4P5O​
Identifiers
Symbols	UBE2M ; UBC-RS2; UBC12; hUbc12
External IDs	OMIM: 603173 MGI: 108278 HomoloGene: 2952 GeneCards: UBE2M Gene
EC number	6.3.2.19
Gene ontology Molecular function • ubiquitin-protein transferase activity • protein binding • ATP binding • ribosomal S6-glutamic acid ligase activity • NEDD8 transferase activity • ubiquitin protein ligase binding • ubiquitin protein ligase activity Cellular component • cytoplasm • cytosol • extracellular exosome Biological process • stimulatory C-type lectin receptor signaling pathway • cellular protein modification process • transforming growth factor beta receptor signaling pathway • protein ubiquitination • positive regulation of neuron apoptotic process • innate immune response • protein neddylation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9040	22192
Ensembl	ENSG00000130725	ENSMUSG00000005575
UniProt	P61081	P61082
RefSeq (mRNA)	NM_003969	NM_001168469
RefSeq (protein)	NP_003960	NP_001161941
Location (UCSC)	Chr 19: 58.56 – 58.56 Mb	Chr 7: 13.04 – 13.04 Mb
PubMed search

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.^[1][2]

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.^[2]

Interactions

UBE2M has been shown to interact with NEDD8,^[3] PRKAR1A^[4] and UBA3.^[5]

References

1. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (Aug 1998). "A new NEDD8-ligating system for cullin-4A". Genes Dev 12 (15): 2263–8. doi:10.1101/gad.12.15.2263. PMC 317039. PMID 9694792.
2. "Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)".
3. Gong, L; Yeh E T (Apr 1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. (UNITED STATES) 274 (17): 12036–42. doi:10.1074/jbc.274.17.12036. ISSN 0021-9258. PMID 10207026.
4. Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Cite uses deprecated parameter |coauthors= (help)
5. Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA (February 2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.

Further reading

• Gong L, Yeh ET (1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17): 12036–42. doi:10.1074/jbc.274.17.12036. PMID 10207026.
• Chen Y, McPhie DL, Hirschberg J, Neve RL (2000). "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons". J. Biol. Chem. 275 (12): 8929–35. doi:10.1074/jbc.275.12.8929. PMID 10722740.
• Wada H, Yeh ET, Kamitani T (2000). "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo". J. Biol. Chem. 275 (22): 17008–15. doi:10.1074/jbc.275.22.17008. PMID 10828074.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Tomsig JL, Snyder SL, Creutz CE (2003). "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif". J. Biol. Chem. 278 (12): 10048–54. doi:10.1074/jbc.M212632200. PMID 12522145.
• Huang DT, Miller DW, Mathew R, et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8". Nat. Struct. Mol. Biol. 11 (10): 927–35. doi:10.1038/nsmb826. PMC 2862556. PMID 15361859.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Huang DT, Paydar A, Zhuang M, et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336.
• Li T, Santockyte R, Shen RF, et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. doi:10.1016/j.abb.2006.03.002. PMID 16620772.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.