NIN (gene)

Ninein (GSK3B interacting protein)
Identifiers
Symbols	NIN ; SCKL7
External IDs	OMIM: 608684 MGI: 105108 HomoloGene: 40632 GeneCards: NIN Gene
Gene ontology Molecular function • calcium ion binding • protein binding • GTP binding Cellular component • spindle pole • nucleolus • centrosome • centriole • microtubule Biological process • microtubule anchoring at centrosome • centrosome localization • centrosome-templated microtubule nucleation Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	51199	18080
Ensembl	ENSG00000100503	ENSMUSG00000021068
UniProt	Q8N4C6	Q61043
RefSeq (mRNA)	NM_016350	NM_001081453
RefSeq (protein)	NP_057434	NP_001074922
Location (UCSC)	Chr 14: 50.72 – 50.83 Mb	Chr 12: 70.01 – 70.11 Mb
PubMed search

Ninein is a protein that in humans is encoded by the NIN gene.^[1][2][3] Ninein, together with its paralog Ninein-like protein is one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.^[3]

References

1. Hong YR, Chen CH, Chang JH, Wang S, Sy WD, Chou CK, Howng SL (Oct 2000). "Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta". Biochim Biophys Acta 1492 (2–3): 513–6. doi:10.1016/S0167-4781(00)00127-5. PMID 11004522.
2. Hong YR, Chen CH, Chuo MH, Liou SY, Howng SL (Feb 2001). "Genomic organization and molecular characterization of the human ninein gene". Biochem. Biophys. Res. Commun. 279 (3): 989–95. doi:10.1006/bbrc.2000.4050. PMID 11162463.
3. "Entrez Gene: NIN ninein (GSK3B interacting protein)".

Further reading

• Nakajima D, Okazaki N, Yamakawa H, et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Mack GJ, Rees J, Sandblom O, et al. (1998). "Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome". Arthritis Rheum. 41 (3): 551–8. doi:10.1002/1529-0131(199803)41:3<551::AID-ART22>3.0.CO;2-X. PMID 9506584.
• Nagase T, Kikuno R, Nakayama M, et al. (2001). "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (4): 273–81. doi:10.1093/dnares/7.4.271. PMID 10997877.
• Ou YY, Mack GJ, Zhang M, Rattner JB (2002). "CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation". J. Cell. Sci. 115 (Pt 9): 1825–35. PMID 11956314.
• Dammermann A, Merdes A (2002). "Assembly of centrosomal proteins and microtubule organization depends on PCM-1". J. Cell Biol. 159 (2): 255–66. doi:10.1083/jcb.200204023. PMC 2173044. PMID 12403812.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Chen CH, Howng SL, Cheng TS, et al. (2003). "Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle". Biochem. Biophys. Res. Commun. 308 (4): 975–83. doi:10.1016/S0006-291X(03)01510-9. PMID 12927815.
• Fritzler MJ, Zhang M, Stinton LM, Rattner JB (2004). "Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia". BMC pediatrics 3: 11. doi:10.1186/1471-2431-3-11. PMC 222907. PMID 14503922.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Innocenti M, Zucconi A, Disanza A, et al. (2004). "Abi1 is essential for the formation and activation of a WAVE2 signalling complex". Nat. Cell Biol. 6 (4): 319–27. doi:10.1038/ncb1105. PMID 15048123.
• Vizmanos JL, Novo FJ, Román JP, et al. (2004). "NIN, a gene encoding a CEP110-like centrosomal protein, is fused to PDGFRB in a patient with a t(5;14)(q33;q24) and an imatinib-responsive myeloproliferative disorder". Cancer Res. 64 (8): 2673–6. doi:10.1158/0008-5472.CAN-04-0144. PMID 15087377.
• Howng SL, Hsu HC, Cheng TS, et al. (2004). "A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3beta and is highly expressed in brain tumors". FEBS Lett. 566 (1–3): 162–8. doi:10.1016/j.febslet.2004.04.024. PMID 15147888.
• Stillwell EE, Zhou J, Joshi HC (2006). "Human ninein is a centrosomal autoantigen recognized by CREST patient sera and plays a regulatory role in microtubule nucleation". Cell Cycle 3 (7): 923–30. doi:10.4161/cc.3.7.947. PMID 15190203.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Delgehyr N, Sillibourne J, Bornens M (2005). "Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function". J. Cell. Sci. 118 (Pt 8): 1565–75. doi:10.1242/jcs.02302. PMID 15784680.
• Cheng TS, Chang LK, Howng SL, et al. (2006). "SUMO-1 modification of centrosomal protein hNinein promotes hNinein nuclear localization". Life Sci. 78 (10): 1114–20. doi:10.1016/j.lfs.2005.06.021. PMID 16154161.