POLE4

Polymerase (DNA-directed), epsilon 4, accessory subunit

Identifiers

POLE4 ; YHHQ1; p12

External IDs

OMIM: 607269 HomoloGene: 41339 GeneCards: POLE4 Gene

2.7.7.7

Gene ontology
Molecular function	• DNA-directed DNA polymerase activity • protein binding • sequence-specific DNA binding • protein heterodimerization activity
Cellular component	• nucleus • Ada2/Gcn5/Ada3 transcription activator complex • epsilon DNA polymerase complex
Biological process	• histone H3 acetylation • DNA biosynthetic process
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 2:
74.96 – 74.97 Mb

Chr 6:
82.62 – 82.71 Mb

PubMed search

Polymerase (DNA-directed), epsilon 4, accessory subunit is a protein that in humans is encoded by the POLE4 gene.^[1]

Function

POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.^[1]

References

1 2 "Entrez Gene: Polymerase (DNA-directed), epsilon 4, accessory subunit".

Post SM, Tomkinson AE, Lee EY (2003). "The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase epsilon". Nucleic Acids Res. 31 (19): 5568–75. doi:10.1093/nar/gkg765. PMC 206465. PMID 14500819.
Li Y, Pursell ZF, Linn S (2000). "Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon". J. Biol. Chem. 275 (30): 23247–52. doi:10.1074/jbc.M002548200. PMID 10801849.
Wang YL, Faiola F, Xu M, Pan S, Martinez E (2008). "Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein". J. Biol. Chem. 283 (49): 33808–15. doi:10.1074/jbc.M806936200. PMC 2590711. PMID 18838386.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

DNA replication (comparing Prokaryotic to Eukaryotic)

Initiation

Prokaryotic (initiation)	Pre-replication complex dnaC Cdc6 Helicase dnaA dnaB T7 Primase dnaG

Eukaryotic (preparation in G1 phase)	Pre-replication complex Origin recognition complex ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 Cdc6 Cdt1 Minichromosome maintenance MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 Licensing factor Autonomously replicating sequence Single-strand binding protein SSBP2 SSBP3 SSBP4 RNase H RNASEH1 RNASEH2A Helicase: HFM1 Primase: PRIM1 PRIM2

Both	Origin of replication/Ori/Replicon Replication fork Lagging and leading strands Okazaki fragments Primer

Replication

Prokaryotic (elongation)	DNA polymerase III holoenzyme dnaC dnaE dnaH dnaN dnaQ dnaT dnaX holA holB holC holD holE Replisome DNA ligase DNA clamp Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment

Eukaryotic (synthesis in S phase)	Replication factor C RFC1 Flap endonuclease FEN1 Topoisomerase Replication protein A RPA1 Eukaryotic DNA polymerase: alpha POLA1 POLA2 PRIM1 PRIM2 delta POLD1 POLD2 POLD3 POLD4 epsilon POLE POLE2 POLE3 POLE4 DNA clamp PCNA Control of chromosome duplication

Both	Movement: Processivity DNA ligase

Termination

Telomere: Telomerase
- TERT
- TERC
- DKC1

This article is issued from Wikipedia - version of the Sunday, March 22, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.

POLE4

Function

References

Further Reading