PRIM2

Primase, DNA, polypeptide 2 (58kDa)
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3L9Q​, 3Q36​, 4BPU​, 4BPW​, 4BPX​, 4RR2​
Identifiers
Symbols	PRIM2 ; PRIM2A; p58
External IDs	OMIM: 176636 MGI: 97758 HomoloGene: 731 GeneCards: PRIM2 Gene
EC number	2.7.7.-
Gene ontology Molecular function • DNA binding • DNA primase activity • metal ion binding • 4 iron, 4 sulfur cluster binding Cellular component • nucleoplasm • primosome complex Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • telomere maintenance via recombination • telomere maintenance • DNA replication, synthesis of RNA primer • DNA replication initiation • DNA strand elongation involved in DNA replication • telomere maintenance via semi-conservative replication Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5558	19076
Ensembl	ENSG00000146143	ENSMUSG00000026134
UniProt	P49643	P33610
RefSeq (mRNA)	NM_000947	NM_008922
RefSeq (protein)	NP_000938	NP_032948
Location (UCSC)	Chr 6: 57.31 – 57.65 Mb	Chr 1: 33.45 – 33.67 Mb
PubMed search

DNA primase large subunit is an enzyme that in humans is encoded by the PRIM2 gene.^[1][2]

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit.^[2]

References

1. Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H; et al. (Feb 1996). "Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12". Genomics 28 (2): 350–3. doi:10.1006/geno.1995.1155. PMID 8530050.
2. "Entrez Gene: PRIM2A primase, polypeptide 2A, 58kDa".

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, et al. (1994). "DNA replication in vitro by recombinant DNA-polymerase-alpha-primase.". Eur. J. Biochem. 222 (3): 781–93. doi:10.1111/j.1432-1033.1994.tb18925.x. PMID 8026492.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Coll JM, Hickey RJ, Cronkey EA, et al. (1998). "Mapping specific protein-protein interactions within the core component of the breast cell DNA synthesome". Oncol. Res. 9 (11–12): 629–39. PMID 9563011.
• Schneider A, Smith RW, Kautz AR, et al. (1998). "Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit". J. Biol. Chem. 273 (34): 21608–15. doi:10.1074/jbc.273.34.21608. PMID 9705292.
• Arezi B, Kirk BW, Copeland WC, Kuchta RD (1999). "Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit". Biochemistry 38 (39): 12899–907. doi:10.1021/bi9908991. PMID 10504261.
• Smith RW, Nasheuer HP (2002). "Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180". FEBS Lett. 527 (1–3): 143–6. doi:10.1016/S0014-5793(02)03197-6. PMID 12220650.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature 425 (6960): 805–11. doi:10.1038/nature02055. PMID 14574404.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.