PRIM1

Primase, DNA, polypeptide 1 (49kDa)
Identifiers
Symbols	PRIM1 MGC12308; p49
External IDs	OMIM: 176635 MGI: 97757 HomoloGene: 730 GeneCards: PRIM1 Gene
EC number	2.7.7.7
Gene ontology Molecular function • DNA binding • DNA primase activity • metal ion binding • 4 iron, 4 sulfur cluster binding Cellular component • nucleoplasm • primosome complex Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • telomere maintenance via recombination • telomere maintenance • DNA replication, synthesis of RNA primer • DNA replication initiation • DNA strand elongation involved in DNA replication • telomere maintenance via semi-conservative replication Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5557	19075
Ensembl	ENSG00000198056	ENSMUSG00000025395
UniProt	P49642	n/a
RefSeq (mRNA)	NM_000946.2	NM_008921.2
RefSeq (protein)	NP_000937.1	NP_032947.1
Location (UCSC)	Chr 12: 54.19 – 54.19 Mb	n/a
PubMed search

DNA primase small subunit is an enzyme that in humans is encoded by the PRIM1 gene.^[1]

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

References

1. Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H; et al. (Feb 1996). "Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12". Genomics 28 (2): 350–3. doi:10.1006/geno.1995.1155. PMID 8530050.

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, et al. (1994). "DNA replication in vitro by recombinant DNA-polymerase-alpha-primase.". Eur. J. Biochem. 222 (3): 781–93. doi:10.1111/j.1432-1033.1994.tb18925.x. PMID 8026492.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Schneider A, Smith RW, Kautz AR, et al. (1998). "Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit". J. Biol. Chem. 273 (34): 21608–15. doi:10.1074/jbc.273.34.21608. PMID 9705292.
• Arezi B, Kirk BW, Copeland WC, Kuchta RD (1999). "Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit". Biochemistry 38 (39): 12899–907. doi:10.1021/bi9908991. PMID 10504261.
• Smith RW, Nasheuer HP (2002). "Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180". FEBS Lett. 527 (1–3): 143–6. doi:10.1016/S0014-5793(02)03197-6. PMID 12220650.