ATP2A1

ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
PDB rendering based on 1iwo.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1iwo​, 1kju​, 1su4​, 1t5s​, 1t5t​, 1vfp​, 1wpe​, 1wpg​, 1xp5​, 2agv​, 2by4​, 2c88​, 2c8k​, 2c8l​, 2c9m​, 2dqs​, 2ear​, 2eas​, 2eat​, 2eau​, 2o9j​, 2oa0​
Identifiers
Symbols	ATP2A1 ; ATP2A; SERCA1
External IDs	OMIM: 108730 MGI: 105058 HomoloGene: 7635 ChEMBL: 3136 GeneCards: ATP2A1 Gene
EC number	3.6.3.8
Gene ontology Molecular function • calcium-transporting ATPase activity • calcium ion binding • protein binding • ATP binding • protein homodimerization activity Cellular component • mitochondrion • endoplasmic reticulum membrane • endoplasmic reticulum-Golgi intermediate compartment • membrane • integral component of membrane • sarcoplasmic reticulum • platelet dense tubular network membrane • H zone • I band • sarcoplasmic reticulum membrane • calcium channel complex • perinuclear region of cytoplasm Biological process • calcium ion transport • regulation of striated muscle contraction • blood coagulation • metabolic process • apoptotic mitochondrial changes • positive regulation of fast-twitch skeletal muscle fiber contraction • positive regulation of endoplasmic reticulum calcium ion concentration • negative regulation of endoplasmic reticulum calcium ion concentration • ion transmembrane transport • response to endoplasmic reticulum stress • negative regulation of striated muscle contraction • positive regulation of mitochondrial calcium ion concentration • maintenance of mitochondrion location • transmembrane transport • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress • calcium ion import • calcium ion transmembrane transport • relaxation of skeletal muscle Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	487	11937
Ensembl	ENSG00000196296	ENSMUSG00000030730
UniProt	O14983	Q8R429
RefSeq (mRNA)	NM_001286075	NM_007504
RefSeq (protein)	NP_001273004	NP_031530
Location (UCSC)	Chr 16: 28.88 – 28.9 Mb	Chr 7: 126.45 – 126.46 Mb
PubMed search

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 is an enzyme that in humans is encoded by the ATP2A1 gene.^[1]

Function

This gene encodes one of the SERCA Ca²⁺-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction.^[1]

Clinical significance

Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.^[1]

Interactions

ATP2A1 has been shown to interact with:

• SLN,^[2][3] and
• PLN.^[2][4][5]

References

1. "Entrez Gene: ATP2A1 ATPase, Ca⁺⁺ transporting, cardiac muscle, fast twitch 1".
2. Asahi M, Kurzydlowski K, Tada M, MacLennan DH (July 2002). "Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs)". J. Biol. Chem. 277 (30): 26725–8. doi:10.1074/jbc.C200269200. PMID 12032137.
3. Asahi M, Sugita Y, Kurzydlowski K, De Leon S, Tada M, Toyoshima C, MacLennan DH (April 2003). "Sarcolipin regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholamban". Proc. Natl. Acad. Sci. U.S.A. 100 (9): 5040–5. doi:10.1073/pnas.0330962100. PMC 154294. PMID 12692302.
4. Asahi M, Kimura Y, Kurzydlowski K, Tada M, MacLennan DH (November 1999). "Transmembrane helix M6 in sarco(endo)plasmic reticulum Ca(2+)-ATPase forms a functional interaction site with phospholamban. Evidence for physical interactions at other sites". J. Biol. Chem. 274 (46): 32855–62. doi:10.1074/jbc.274.46.32855. PMID 10551848.
5. Asahi M, Green NM, Kurzydlowski K, Tada M, MacLennan DH (August 2001). "Phospholamban domain IB forms an interaction site with the loop between transmembrane helices M6 and M7 of sarco(endo)plasmic reticulum Ca2+ ATPases". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10061–6. doi:10.1073/pnas.181348298. PMC 56915. PMID 11526231.

Further reading

• Baba-Aissa F, Raeymaekers L, Wuytack F; et al. (1998). "Distribution and isoform diversity of the organellar Ca²⁺ pumps in the brain.". Mol. Chem. Neuropathol. 33 (3): 199–208. doi:10.1007/BF02815182. PMID 9642673.
• Callen DF, Baker E, Lane S; et al. (1992). "Regional mapping of the Batten disease locus (CLN3) to human chromosome 16p12.". Am. J. Hum. Genet. 49 (6): 1372–7. PMC 1702406. PMID 1746562.
• MacLennan DH, Brandl CJ, Champaneria S; et al. (1988). "Fast-twitch and slow-twitch/cardiac Ca²⁺ ATPase genes map to human chromosomes 16 and 12.". Somat. Cell Mol. Genet. 13 (4): 341–6. doi:10.1007/BF01534928. PMID 2842876.
• Brandl CJ, Green NM, Korczak B, MacLennan DH (1986). "Two Ca²⁺ ATPase genes: homologies and mechanistic implications of deduced amino acid sequences.". Cell 44 (4): 597–607. doi:10.1016/0092-8674(86)90269-2. PMID 2936465.
• Benders AA, Wevers RA, Veerkamp JH (1996). "Ion transport in human skeletal muscle cells: disturbances in myotonic dystrophy and Brody's disease.". Acta Physiol. Scand. 156 (3): 355–67. doi:10.1046/j.1365-201X.1996.202000.x. PMID 8729696.
• Zhang Y, Fujii J, Phillips MS; et al. (1997). "Characterization of cDNA and genomic DNA encoding SERCA1, the Ca²⁺-ATPase of human fast-twitch skeletal muscle sarcoplasmic reticulum, and its elimination as a candidate gene for Brody disease.". Genomics 30 (3): 415–24. doi:10.1006/geno.1995.1259. PMID 8825625.
• Odermatt A, Taschner PE, Khanna VK; et al. (1996). "Mutations in the gene-encoding SERCA1, the fast-twitch skeletal muscle sarcoplasmic reticulum Ca²⁺ ATPase, are associated with Brody disease.". Nat. Genet. 14 (2): 191–4. doi:10.1038/ng1096-191. PMID 8841193.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Algenstaedt P, Antonetti DA, Yaffe MB, Kahn CR (1997). "Insulin receptor substrate proteins create a link between the tyrosine phosphorylation cascade and the Ca²⁺-ATPases in muscle and heart.". J. Biol. Chem. 272 (38): 23696–702. doi:10.1074/jbc.272.38.23696. PMID 9295312.
• Odermatt A, Taschner PE, Scherer SW; et al. (1998). "Characterization of the gene encoding human sarcolipin (SLN), a proteolipid associated with SERCA1: absence of structural mutations in five patients with Brody disease.". Genomics 45 (3): 541–53. doi:10.1006/geno.1997.4967. PMID 9367679.
• MacLennan DH, Rice WJ, Odermatt A (1998). "Structure/function analysis of the Ca²⁺ binding and translocation domain of SERCA1 and the role in Brody disease of the ATP2A1 gene encoding SERCA1.". Ann. N. Y. Acad. Sci. 834: 175–85. doi:10.1111/j.1749-6632.1997.tb52249.x. PMID 9405806.
• Odermatt A, Becker S, Khanna VK; et al. (1998). "Sarcolipin regulates the activity of SERCA1, the fast-twitch skeletal muscle sarcoplasmic reticulum Ca²⁺-ATPase.". J. Biol. Chem. 273 (20): 12360–9. doi:10.1074/jbc.273.20.12360. PMID 9575189.
• Asahi M, Kimura Y, Kurzydlowski K; et al. (2000). "Transmembrane helix M6 in sarco(endo)plasmic reticulum Ca²⁺-ATPase forms a functional interaction site with phospholamban. Evidence for physical interactions at other sites.". J. Biol. Chem. 274 (46): 32855–62. doi:10.1074/jbc.274.46.32855. PMID 10551848.
• Odermatt A, Barton K, Khanna VK; et al. (2000). "The mutation of Pro789 to Leu reduces the activity of the fast-twitch skeletal muscle sarco(endo)plasmic reticulum Ca²⁺ ATPase (SERCA1) and is associated with Brody disease.". Hum. Genet. 106 (5): 482–91. doi:10.1007/s004390000297. PMID 10914677.
• Daiho T, Yamasaki K, Saino T; et al. (2001). "Mutations of either or both Cys876 and Cys888 residues of sarcoplasmic reticulum Ca²⁺ATPase result in a complete loss of Ca²⁺ transport activity without a loss of Ca²⁺-dependent ATPase activity. Role of the CYS876-CYS888 disulfide bond.". J. Biol. Chem. 276 (35): 32771–8. doi:10.1074/jbc.M101229200. PMID 11438520.
• Asahi M, Green NM, Kurzydlowski K; et al. (2001). "Phospholamban domain IB forms an interaction site with the loop between transmembrane helices M6 and M7 of sarco(endo)plasmic reticulum Ca²⁺ ATPases.". Proc. Natl. Acad. Sci. U.S.A. 98 (18): 10061–6. doi:10.1073/pnas.181348298. PMC 56915. PMID 11526231.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Pieske B, Maier LS, Schmidt-Schweda S (2002). "Sarcoplasmic reticulum Ca²⁺ load in human heart failure.". Basic Res. Cardiol. 97 Suppl 1: I63–71. PMID 12479237.
• Toyoshima C, Asahi M, Sugita Y; et al. (2003). "Modeling of the inhibitory interaction of phospholamban with the Ca²⁺ ATPase.". Proc. Natl. Acad. Sci. U.S.A. 100 (2): 467–72. doi:10.1073/pnas.0237326100. PMC 141018. PMID 12525698.