Eukaryotic translation initiation factor 4 gamma

Eukaryotic translation initiation factor 4 gamma, 1
PDB rendering based on 1ug3.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1LJ2​, 1UG3​, 2W97​, 4AZA​, 4F02​
Identifiers
Symbols	EIF4G1 ; EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
External IDs	OMIM: 600495 MGI: 2384784 HomoloGene: 110725 ChEMBL: 1741197 GeneCards: EIF4G1 Gene
Gene ontology Molecular function • translation initiation factor activity • protein binding • translation factor activity, RNA binding • eukaryotic initiation factor 4E binding • poly(A) RNA binding Cellular component • cytoplasm • cytosol • membrane • eukaryotic translation initiation factor 4F complex Biological process • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • behavioral fear response • translation • translational initiation • regulation of translational initiation • insulin receptor signaling pathway • gene expression • viral process • cytokine-mediated signaling pathway • cellular protein metabolic process • positive regulation of neuron differentiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1981	208643
Ensembl	ENSG00000114867	ENSMUSG00000045983
UniProt	Q04637	Q6NZJ6
RefSeq (mRNA)	NM_001194946	NM_001005331
RefSeq (protein)	NP_001181875	NP_001005331
Location (UCSC)	Chr 3: 184.31 – 184.34 Mb	Chr 16: 20.67 – 20.69 Mb
PubMed search

Eukaryotic translation initiation factor 4 gamma 1 is a protein that in humans is encoded by the EIF4G1 gene.^[1][2]

Function

The protein encoded by this gene is a component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.^[3] eIF4G serves as a scaffold, interacting with mRNA and the other components of the eIF4F complex, as well as the PABP and eIF3.

Interactions

Eukaryotic translation initiation factor 4 gamma has been shown to interact with MKNK1,^[4] EIF4A1,^[5][6][7] EIF4E,^{[6][7][8][9][10]} MKNK2^[11] and PABPC1.^[12]

See also

• Eukaryotic initiation factor

References

1. Yan R, Rychlik W, Etchison D, Rhoads RE (Nov 1992). "Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma". The Journal of Biological Chemistry 267 (32): 23226–31. PMID 1429670.
2. Imataka H, Sonenberg N (Dec 1997). "Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A". Molecular and Cellular Biology 17 (12): 6940–7. PMC 232551. PMID 9372926.
3. "Entrez Gene: EIF4G1 eukaryotic translation initiation factor 4 gamma, 1".
4. Pyronnet S, Imataka H, Gingras AC, Fukunaga R, Hunter T, Sonenberg N (Jan 1999). "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E". The EMBO Journal 18 (1): 270–9. doi:10.1093/emboj/18.1.270. PMC 1171121. PMID 9878069.
5. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
6. Connolly E, Braunstein S, Formenti S, Schneider RJ (May 2006). "Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells". Molecular and Cellular Biology 26 (10): 3955–65. doi:10.1128/MCB.26.10.3955-3965.2006. PMC 1489005. PMID 16648488.
7. Harris TE, Chi A, Shabanowitz J, Hunt DF, Rhoads RE, Lawrence JC (Apr 2006). "mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin". The EMBO Journal 25 (8): 1659–68. doi:10.1038/sj.emboj.7601047. PMC 1440840. PMID 16541103.
8. Vary TC, Jefferson LS, Kimball SR (Dec 1999). "Amino acid-induced stimulation of translation initiation in rat skeletal muscle". The American Journal of Physiology 277 (6 Pt 1): E1077–86. PMID 10600798.
9. Mader S, Lee H, Pause A, Sonenberg N (Sep 1995). "The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins". Molecular and Cellular Biology 15 (9): 4990–7. PMC 230746. PMID 7651417.
10. Kumar V, Sabatini D, Pandey P, Gingras AC, Majumder PK, Kumar M, Yuan ZM, Carmichael G, Weichselbaum R, Sonenberg N, Kufe D, Kharbanda S (Apr 2000). "Regulation of the rapamycin and FKBP-target 1/mammalian target of rapamycin and cap-dependent initiation of translation by the c-Abl protein-tyrosine kinase". The Journal of Biological Chemistry 275 (15): 10779–87. doi:10.1074/jbc.275.15.10779. PMID 10753870.
11. Scheper GC, Parra JL, Wilson M, Van Kollenburg B, Vertegaal AC, Han ZG, Proud CG (Aug 2003). "The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization". Molecular and Cellular Biology 23 (16): 5692–705. doi:10.1128/MCB.23.16.5692-5705.2003. PMC 166352. PMID 12897141.
12. Imataka H, Gradi A, Sonenberg N (Dec 1998). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation". The EMBO Journal 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.

Further reading

• Gingras AC, Raught B, Sonenberg N (2000). "eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation". Annual Review of Biochemistry 68: 913–63. doi:10.1146/annurev.biochem.68.1.913. PMID 10872469.
• Yan R, Rhoads RE (Mar 1995). "Human protein synthesis initiation factor eIF-4 gamma is encoded by a single gene (EIF4G) that maps to chromosome 3q27-qter". Genomics 26 (2): 394–8. doi:10.1016/0888-7543(95)80227-D. PMID 7601469.
• Mader S, Lee H, Pause A, Sonenberg N (Sep 1995). "The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins". Molecular and Cellular Biology 15 (9): 4990–7. PMC 230746. PMID 7651417.
• Pause A, Belsham GJ, Gingras AC, Donzé O, Lin TA, Lawrence JC, Sonenberg N (Oct 1994). "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function". Nature 371 (6500): 762–7. doi:10.1038/371762a0. PMID 7935836.
• Lamphear BJ, Yan R, Yang F, Waters D, Liebig HD, Klump H, Kuechler E, Skern T, Rhoads RE (Sep 1993). "Mapping the cleavage site in protein synthesis initiation factor eIF-4 gamma of the 2A proteases from human Coxsackievirus and rhinovirus". The Journal of Biological Chemistry 268 (26): 19200–3. PMID 8396129.
• Haghighat A, Mader S, Pause A, Sonenberg N (Nov 1995). "Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E". The EMBO Journal 14 (22): 5701–9. PMC 394685. PMID 8521827.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Lamphear BJ, Rhoads RE (Dec 1996). "A single amino acid change in protein synthesis initiation factor 4G renders cap-dependent translation resistant to picornaviral 2A proteases". Biochemistry 35 (49): 15726–33. doi:10.1021/bi961864t. PMID 8961935.
• Brass N, Heckel D, Sahin U, Pfreundschuh M, Sybrecht GW, Meese E (Jan 1997). "Translation initiation factor eIF-4gamma is encoded by an amplified gene and induces an immune response in squamous cell lung carcinoma". Human Molecular Genetics 6 (1): 33–9. doi:10.1093/hmg/6.1.33. PMID 9002667.
• Eberle J, Krasagakis K, Orfanos CE (May 1997). "Translation initiation factor eIF-4A1 mRNA is consistently overexpressed in human melanoma cells in vitro". International Journal of Cancer. Journal International Du Cancer 71 (3): 396–401. doi:10.1002/(SICI)1097-0215(19970502)71:3<396::AID-IJC16>3.0.CO;2-E. PMID 9139875.
• Gradi A, Imataka H, Svitkin YV, Rom E, Raught B, Morino S, Sonenberg N (Jan 1998). "A novel functional human eukaryotic translation initiation factor 4G". Molecular and Cellular Biology 18 (1): 334–42. PMC 121501. PMID 9418880.
• Piron M, Vende P, Cohen J, Poncet D (Oct 1998). "Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F". The EMBO Journal 17 (19): 5811–21. doi:10.1093/emboj/17.19.5811. PMC 1170909. PMID 9755181.
• Imataka H, Gradi A, Sonenberg N (Dec 1998). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation". The EMBO Journal 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
• Pyronnet S, Imataka H, Gingras AC, Fukunaga R, Hunter T, Sonenberg N (Jan 1999). "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E". The EMBO Journal 18 (1): 270–9. doi:10.1093/emboj/18.1.270. PMC 1171121. PMID 9878069.
• Laroia G, Cuesta R, Brewer G, Schneider RJ (Apr 1999). "Control of mRNA decay by heat shock-ubiquitin-proteasome pathway". Science 284 (5413): 499–502. doi:10.1126/science.284.5413.499. PMID 10205060.
• Vary TC, Jefferson LS, Kimball SR (Dec 1999). "Amino acid-induced stimulation of translation initiation in rat skeletal muscle". The American Journal of Physiology 277 (6 Pt 1): E1077–86. PMID 10600798.
• Cuesta R, Laroia G, Schneider RJ (Jun 2000). "Chaperone hsp27 inhibits translation during heat shock by binding eIF4G and facilitating dissociation of cap-initiation complexes". Genes & Development 14 (12): 1460–70. PMC 316692. PMID 10859165.
• Chartier-Harlin MC, Dachsel JC, Vilariño-Güell C, Lincoln SJ, Leprêtre F, Hulihan MM, Kachergus J, Milnerwood AJ, Tapia L, Song MS, Le Rhun E, Mutez E, Larvor L, Duflot A, Vanbesien-Mailliot C, Kreisler A, Ross OA, Nishioka K, Soto-Ortolaza AI, Cobb SA, Melrose HL, Behrouz B, Keeling BH, Bacon JA, Hentati E, Williams L, Yanagiya A, Sonenberg N, Lockhart PJ, Zubair AC, Uitti RJ, Aasly JO, Krygowska-Wajs A, Opala G, Wszolek ZK, Frigerio R, Maraganore DM, Gosal D, Lynch T, Hutchinson M, Bentivoglio AR, Valente EM, Nichols WC, Pankratz N, Foroud T, Gibson RA, Hentati F, Dickson DW, Destée A, Farrer MJ (Sep 2011). "Translation initiator EIF4G1 mutations in familial Parkinson disease". American Journal of Human Genetics 89 (3): 398–406. doi:10.1016/j.ajhg.2011.08.009. PMID 21907011.