Replication protein A1

Replication protein A1, 70kDa
PDB rendering based on 1ewi.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1EWI​, 1FGU​, 1JMC​, 1L1O​, 2B29​, 2B3G​, 4IJH​, 4IJL​, 4IPC​, 4IPD​, 4IPG​, 4IPH​, 4LUO​, 4LUV​, 4LUZ​, 4LW1​, 4LWC​, 4NB3​, 4O0A​, 4R4C​, 4R4I​, 4R4O​, 4R4Q​, 4R4T​
Identifiers
Symbols	RPA1 ; HSSB; MST075; REPA1; RF-A; RP-A; RPA70
External IDs	OMIM: 179835 MGI: 1915525 HomoloGene: 2208 ChEMBL: 1764940 GeneCards: RPA1 Gene
Gene ontology Molecular function • chromatin binding • damaged DNA binding • single-stranded DNA binding • protein binding • metal ion binding Cellular component • lateral element • male germ cell nucleus • nucleus • nucleoplasm • DNA replication factor A complex • PML body Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • nucleotide-excision repair, DNA damage removal • telomere maintenance via recombination • telomere maintenance • double-strand break repair via homologous recombination • DNA recombinase assembly • in utero embryonic development • DNA replication • DNA-dependent DNA replication • DNA strand elongation involved in DNA replication • DNA repair • transcription-coupled nucleotide-excision repair • base-excision repair • nucleotide-excision repair • nucleotide-excision repair, DNA gap filling • mismatch repair • double-strand break repair • DNA recombination • meiotic nuclear division • positive regulation of cell proliferation • protein sumoylation • translesion synthesis • hemopoiesis • telomere maintenance via semi-conservative replication • cellular response to heat • error-prone translesion synthesis • DNA damage response, detection of DNA damage • post-translational protein modification • cellular protein metabolic process • homeostasis of number of cells within a tissue • error-free translesion synthesis • regulation of cellular response to heat Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	6117	68275
Ensembl	ENSG00000132383	ENSMUSG00000000751
UniProt	P27694	Q8VEE4
RefSeq (mRNA)	NM_002945	NM_001164223
RefSeq (protein)	NP_002936	NP_001157695
Location (UCSC)	Chr 17: 1.83 – 1.9 Mb	Chr 11: 75.3 – 75.35 Mb
PubMed search

Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.^[1]

Interactions

Replication protein A1 has been shown to interact with:

• BRCA2,^[2]
• BLM,^[3][4][5]
• MCM2,^[6]
• MCM4,^[6]
• MCM6,^[6]
• MCM7,^[6]
• MUTYH,^[7]
• ORC2L,^[6]
• ORC6L,^[6]
• P53,^[8][9]
• RPA2,^[10][11]
• RPA3,^[11][12]
• TIPIN,^[13]
• TP53BP1,^[14] and
• XPA.^[15]

References

1. Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (April 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". J Biol Chem 268 (9): 6131–8. PMID 8454588.
2. Wong JM, Ionescu D, Ingles CJ (January 2003). "Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2". Oncogene 22 (1): 28–33. doi:10.1038/sj.onc.1206071. PMID 12527904.
3. Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (August 2000). "Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity". J. Biol. Chem. 275 (31): 23500–8. doi:10.1074/jbc.M001557200. PMID 10825162.
4. Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002). "Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases". J. Biol. Chem. 277 (43): 41110–9. doi:10.1074/jbc.M205396200. PMID 12181313.
5. Moens PB, Kolas NK, Tarsounas M, Marcon E, Cohen PE, Spyropoulos B (April 2002). "The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination". J. Cell. Sci. 115 (Pt 8): 1611–22. PMID 11950880.
6. Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
7. Parker A, Gu Y, Mahoney W, Lee SH, Singh KK, Lu AL (February 2001). "Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair". J. Biol. Chem. 276 (8): 5547–55. doi:10.1074/jbc.M008463200. PMID 11092888.
8. Romanova LY, Willers H, Blagosklonny MV, Powell SN (Dec 2004). "The interaction of p53 with replication protein A mediates suppression of homologous recombination". Oncogene 23 (56): 9025–33. doi:10.1038/sj.onc.1207982. PMID 15489903.
9. Riva F, Zuco V, Vink AA, Supino R, Prosperi E (Dec 2001). "UV-induced DNA incision and proliferating cell nuclear antigen recruitment to repair sites occur independently of p53-replication protein A interaction in p53 wild type and mutant ovarian carcinoma cells". Carcinogenesis 22 (12): 1971–8. doi:10.1093/carcin/22.12.1971. PMID 11751427.
10. Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (March 1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". EMBO J. 18 (5): 1397–406. doi:10.1093/emboj/18.5.1397. PMC 1171229. PMID 10064605.
11. Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (April 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. 21 (7): 1855–63. doi:10.1093/emboj/21.7.1855. PMC 125950. PMID 11927569.
12. Bochkareva E, Frappier L, Edwards AM, Bochkarev A (February 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. PMID 9461578.
13. Unsal-Kaçmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK (April 2007). "The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement". Mol. Cell. Biol. 27 (8): 3131–42. doi:10.1128/MCB.02190-06. PMC 1899931. PMID 17296725.
14. Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH (August 2005). "53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage". Oncogene 24 (35): 5423–30. doi:10.1038/sj.onc.1208710. PMID 15856006.
15. Li L, Lu X, Peterson CA, Legerski RJ (October 1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair". Mol. Cell. Biol. 15 (10): 5396–402. doi:10.1128/mcb.15.10.5396. PMC 230789. PMID 7565690.

Further reading

• Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB.". Crit. Rev. Biochem. Mol. Biol. 34 (3): 141–80. doi:10.1080/10409239991209255. PMID 10473346.
• Binz SK, Sheehan AM, Wold MS (2005). "Replication protein A phosphorylation and the cellular response to DNA damage.". DNA Repair (Amst.) 3 (8-9): 1015–24. doi:10.1016/j.dnarep.2004.03.028. PMID 15279788.
• Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992). "Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.". EMBO J. 11 (2): 769–76. PMC 556510. PMID 1311258.
• Erdile LF, Heyer WD, Kolodner R, Kelly TJ (1991). "Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.". J. Biol. Chem. 266 (18): 12090–8. PMID 2050703.
• Li L, Lu X, Peterson CA, Legerski RJ (1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair.". Mol. Cell. Biol. 15 (10): 5396–402. doi:10.1128/mcb.15.10.5396. PMC 230789. PMID 7565690.
• Keshav KF, Chen C, Dutta A (1995). "Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex.". Mol. Cell. Biol. 15 (6): 3119–28. doi:10.1128/mcb.15.6.3119. PMC 230543. PMID 7760808.
• Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ (1994). "High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3).". Genomics 20 (2): 249–57. doi:10.1006/geno.1994.1161. PMID 8020972.
• Gomes XV, Wold MS (1996). "Functional domains of the 70-kilodalton subunit of human replication protein A.". Biochemistry 35 (32): 10558–68. doi:10.1021/bi9607517. PMID 8756712.
• Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L (1997). "Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.". Nature 385 (6612): 176–81. doi:10.1038/385176a0. PMID 8990123.
• Amacker M, Hottiger M, Mossi R, Hübscher U (1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase.". AIDS 11 (4): 534–6. PMID 9084803.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.". Mol. Cell. Biol. 17 (4): 1817–23. PMC 232028. PMID 9121429.
• Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (1998). "Identification of DNA replication and cell cycle proteins that interact with PCNA.". Nucleic Acids Res. 25 (24): 5041–6. doi:10.1093/nar/25.24.5041. PMC 147130. PMID 9396813.
• Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain.". J. Biol. Chem. 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. PMID 9461578.
• Golub EI, Gupta RC, Haaf T, Wold MS, Radding CM (1999). "Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA.". Nucleic Acids Res. 26 (23): 5388–93. doi:10.1093/nar/26.23.5388. PMC 148005. PMID 9826763.
• Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.". EMBO J. 18 (5): 1397–406. doi:10.1093/emboj/18.5.1397. PMC 1171229. PMID 10064605.
• Brosh RM, Orren DK, Nehlin JO, Ravn PH, Kenny MK, Machwe A, Bohr VA (1999). "Functional and physical interaction between WRN helicase and human replication protein A.". J. Biol. Chem. 274 (26): 18341–50. doi:10.1074/jbc.274.26.18341. PMID 10373438.
• Kim ST, Lim DS, Canman CE, Kastan MB (2000). "Substrate specificities and identification of putative substrates of ATM kinase family members.". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806.
• Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (2000). "Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity.". J. Biol. Chem. 275 (31): 23500–8. doi:10.1074/jbc.M001557200. PMID 10825162.