Small nucleolar RNA SNORA65

Small nucleolar RNA SNORA65
Predicted secondary structure and sequence conservation of SNORA65
Identifiers
Symbol	SNORA65
Alt. Symbols	U65
Rfam	RF00302
Other data
RNA type	Gene; snRNA; snoRNA; H/ACA-box
Domain(s)	Eukaryota
GO	0006396 0005730
SO	0000594

SNORA65 (also known as U65) is a non-coding RNA (ncRNA) molecule which functions in the biogenesis (modification) of other small nuclear RNAs (snRNAs). This type of modifying RNA is located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a 'guide RNA'.

U65 was originally cloned from HeLa cells ^[1] and belongs to the H/ACA box class of snoRNAs as it has the predicted hairpin-hinge-hairpin-tail structure and has the conserved H/ACA-box motifs.^[2] snoRNA U65 is predicted to guide the pseudouridylation of residues U4373 and U4427 of 28S ribosomal RNA (rRNA).^[3] Pseudouridylation is the isomerisation of the nucleoside uridine to pseudouridine(Ψ). U65 is related to the snoRNA MBII-351 identified in mouse.^[4]

References

1. Ganot, Philippe; Caizergues-Ferrer, Michèle; Kiss, Tamás (1 April 1997). "The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation". Genes & Development 11 (7): 941–956. doi:10.1101/gad.11.7.941. PMID 9106664.
2. Kiss, Tamás (2002). "Small Nucleolar RNAs". Cell 109 (2): 145–148. doi:10.1016/S0092-8674(02)00718-3. PMID 12007400.
3. Entry for U65 at snoRNABase
4. Huttenhofer, Alexander; Kiefmann, Martin; Meier-Ewert, Sebastian; O'Brien, John; Lehrach, Hans; Bachellerie, Jean-Pierre; Brosius, Jürgen (1 June 2001). "RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse". The EMBO Journal 20 (11): 2943–2953. doi:10.1093/emboj/20.11.2943. PMC 125495. PMID 11387227.

External links

• Page for Small nucleolar RNA SNORA65 at Rfam