Phenobarbital

Not to be confused with Pentobarbital.
Phenobarbital
2D chemical structure of phenobarbital
3D ball-and-stick model of phenobarbital
Systematic (IUPAC) name
5-Ethyl-5-phenyl-1,3-diazinane-2,4,6-trione
Clinical data
Trade names Luminal
AHFS/Drugs.com monograph
MedlinePlus a682007
Pregnancy
category
  • AU: D
  • US: D (Evidence of risk)
Dependence
liability		 High
Routes of
administration		 Oral (PO), rectal (PR), parenteral (intramuscular and intravenous)
Legal status
Legal status
Pharmacokinetic data
Bioavailability >95%
Protein binding 20 to 45%
Metabolism Hepatic (mostly CYP2C19)
Onset of action within 5 min (IV) and 30 min (PO)[1]
Biological half-life 53 to 118 hours
Duration of action 4 hrs[1] to 2 days[2]
Excretion Renal and fecal
Identifiers
CAS Number 50-06-6 YesY
ATC code N05CA24 (WHO) N03AA02 (WHO)
PubChem CID 4763
IUPHAR/BPS 2804
DrugBank DB01174 YesY
ChemSpider 4599 YesY
UNII YQE403BP4D YesY
KEGG D00506 YesY
ChEBI CHEBI:8069 YesY
ChEMBL CHEMBL40 YesY
Chemical data
Formula C12H12N2O3
Molar mass 232.235 g/mol
  (verify)

Phenobarbital, also known as phenobarbitone, is a medication recommended by the World Health Organization for the treatment of certain types of epilepsy in developing countries.[3] In the developed world it is commonly used to treat seizures in young children,[4] while other medications are generally used in older children and adults.[5] It may be used intravenously, injected into a muscle, or taken by mouth.[1] The injectable form may be used to treat status epilepticus.[1] Phenobarbital is occasionally used to treat trouble sleeping, anxiety, drug withdrawal, and to help with surgery.[1] It usually begins working within five minutes when used intravenously and half an hour when administered orally.[1] Its effects last for between four hours and two days.[1][2]

Side effects include a decreased level of consciousness along with a decreased effort to breathe. There is concern about both abuse and withdrawal following long term use. It may also increase the risk of suicide.[1] It is pregnancy category B or D in the United States and category D in Australia, meaning that it may cause harm when taken by pregnant women.[1][6] If used during breastfeeding it may result in drowsiness in the baby.[7] A lower dose is recommended in those with poor liver or kidney function, as well as elderly people.[1] Phenobarbital is a barbiturate that works by increasing the activity of the inhibitory neurotransmitter GABA.[1]

Phenobarbital was discovered in 1912 and is the oldest still commonly used anti-seizure medication.[8][9] It is a core medicine in the WHO Model List of Essential Medicines, the minimum medications needed for a basic health care system.[10] It is the least expensive anti-seizure medication at around $5 USD a year.[11] Access however may be difficult as some countries label it as a controlled drug.[11]

Medical uses

Phenobarbital is used in the treatment of all types of seizures except absence seizures.[12][13] It is no less effective at seizure control than more recent drugs such as phenytoin and carbamazepine. It is, however, less well tolerated.[14][15] The World Health Organization gives it a first-line recommendation in the developing world and it is commonly used there.[3][16] Its very long active half-life means for some people doses do not have to be taken every day, particularly once the dose has been stabilized over a period of several weeks or months, and seizures are effectively controlled.

The first-line drugs for treatment of status epilepticus are benzodiazepines, such as lorazepam or diazepam. If these fail, then phenytoin may be used, with phenobarbital being an alternative in the US, but used only third-line in the UK.[17] Failing that, the only treatment is anaesthesia in intensive care.[13][18]

Phenobarbital is the first-line choice for the treatment of neonatal seizures.[19][20][21] Concerns that neonatal seizures in themselves could be harmful make most physicians treat them aggressively. No reliable evidence, though, supports this approach.[22]

Phenobarbital is sometimes used for alcohol detoxification and benzodiazepine detoxification for its sedative and anticonvulsant properties. The benzodiazepines chlordiazepoxide (Librium) and oxazepam (Serax) have largely replaced phenobarbital for detoxification.[23]

Other uses

Phenobarbital properties can effectively reduce tremors and seizures associated with abrupt withdrawal from benzodiazepines.

Phenobarbital is a cytochrome P450 inducer, and is used to reduce the toxicity of some drugs.

Phenobarbital is occasionally prescribed in low doses to aid in the conjugation of bilirubin in people with Crigler-Najjar syndrome (Type II), or in patients with Gilbert syndrome.

Phenobarbital can also be used to relieve cyclic vomiting syndrome symptoms.

Phenobarbital is a commonly used agent in high purity and dosage for lethal injection of "death row" criminals.

In infants suspected of neonatal biliary atresia, phenobarbital is used in preparation for a 99mTc-IDA hepatobiliary study that differentiates atresia from hepatitis or cholestasis.

Phenobarbital is used as a secondary agent to treat newborns with neonatal abstinence syndrome, a condition of withdrawal symptoms from exposure to opioid drugs in utero.

Side effects

Sedation and hypnosis are the principal side effects (occasionally, they are also the intended effects) of phenobarbital. Central nervous system effects, such as dizziness, nystagmus and ataxia, are also common. In elderly patients, it may cause excitement and confusion, while in children, it may result in paradoxical hyperactivity. Another very rare side effect is amelogenesis imperfecta.

Phenobarbital is a cytochrome P450 hepatic enzyme inducer. Drugs that are metabolized by the CYP450 enzyme system will decrease effectiveness because of faster clearance from the system.

Caution is to be used with children. Of anticonvulsant drugs, behavioural disturbances occur most frequently with clonazepam and phenobarbital.[24]

Contraindications

Acute intermittent porphyria, hypersensitivity to any barbiturate, prior dependence on barbiturates, severe respiratory insufficiency and hyperkinesia in children are contraindications for phenobarbital use.

Overdose

Main article: Barbiturate overdose

Phenobarbital causes a depression of the body's systems, mainly the central and peripheral nervous systems; thus, the main characteristic of phenobarbital overdose is a "slowing" of bodily functions, including decreased consciousness (even coma), bradycardia, bradypnea, hypothermia, and hypotension (in massive overdoses). Overdose may also lead to pulmonary edema and acute renal failure as a result of shock, and can result in death.

The electroencephalogram of a person with phenobarbital overdose may show a marked decrease in electrical activity, to the point of mimicking brain death. This is due to profound depression of the central nervous system, and is usually reversible.[25]

Treatment of phenobarbital overdose is supportive, and mainly consists of the maintenance of airway patency (through endotracheal intubation and mechanical ventilation), correction of bradycardia and hypotension (with intravenous fluids and vasopressors, if necessary) and removal of as much drug as possible from the body. Depending on how much time has elapsed since ingestion of the drug, this may be accomplished through gastric lavage (stomach pumping) or use of activated charcoal. Hemodialysis is effective in removing phenobarbital from the body, and may reduce its half-life by up to 90%.[25] No specific antidote for barbiturate poisoning is available.[26]

British veterinarian Donald Sinclair, better known as "Siegfried Farnon" in the "All Creatures Great and Small" books of James Herriot, committed suicide at the age of 84 by injecting himself with an overdose of phenobarbital. Activist Abbie Hoffman also committed suicide by consuming phenobarbital, combined with alcohol, on April 12, 1989; the residue of around 150 pills was found in his body at autopsy.[27] Also dying from an overdose in 1996 was actress/model Margaux Hemingway. The Japanese officers aboard the German submarine U-234 killed themselves with phenobarbital while the German crew members were on their way to the US to surrender (but before Japan had surrendered).

39 members of the Heaven's Gate UFO religious group committed mass suicide in March 1997 by drinking a lethal dose of phenobarbital and vodka "and then lay down to die" hoping to enter an alien spacecraft.[28]

Mechanism of action

The net result is increased flux of chloride ions into the neuron, decreasing excitability. Direct blockade of excitatory glutamate signaling is also believed to contribute to the hypnotic/anticonvulsant effect that is observed with the barbiturates.

Pharmacokinetics

Phenobarbital has an oral bioavailability of about 90%. Peak plasma concentrations are reached eight to 12 hours after oral administration. It is one of the longest-acting barbiturates available – it remains in the body for a very long time (half-life of two to seven days) and has very low protein binding (20 to 45%). Phenobarbital is metabolized by the liver, mainly through hydroxylation and glucuronidation, and induces many isozymes of the cytochrome P450 system. Cytochrome P450 2B6 (CYP2B6) is specifically induced by phenobarbital via the CAR/RXR nuclear receptor heterodimer. It is excreted primarily by the kidneys.

Veterinary uses

Phenobarbital is one of the initial drugs of choice to treat epilepsy in dogs, and is the initial drug of choice to treat epilepsy in cats.[29]

It is also used to treat feline hyperesthesia syndrome in cats when antiobsessional therapies prove ineffective. [30]

It may also be used to treat seizures in horses when benzodiazepine treatment has failed or is contraindicated.[31]

History

The first barbiturate drug, barbital, was synthesized in 1902 by German chemists Emil Fischer and Joseph von Mering and was first marketed as Veronal by Friedr. Bayer et comp. By 1904, several related drugs, including phenobarbital, had been synthesized by Fischer. Phenobarbital was brought to market in 1912 by the drug company Bayer as the brand Luminal. It remained a commonly prescribed sedative and hypnotic until the introduction of benzodiazepines in the 1960s.[32]

Phenobarbital's soporific, sedative and hypnotic properties were well known in 1912, but it was not yet known to be an effective anticonvulsant. The young doctor Alfred Hauptmann[33] gave it to his epilepsy patients as a tranquilizer and discovered their seizures were susceptible to the drug. Hauptmann performed a careful study of his patients over an extended period. Most of these patients were using the only effective drug then available, bromide, which had terrible side effects and limited efficacy. On phenobarbital, their epilepsy was much improved: The worst patients suffered fewer and lighter seizures and some patients became seizure-free. In addition, they improved physically and mentally as bromides were removed from their regimen. Patients who had been institutionalised due to the severity of their epilepsy were able to leave and, in some cases, resume employment. Hauptmann dismissed concerns that its effectiveness in stalling seizures could lead to patients suffering a build-up that needed to be "discharged". As he expected, withdrawal of the drug led to an increase in seizure frequency – it was not a cure. The drug was quickly adopted as the first widely effective anticonvulsant, though World War I delayed its introduction in the U.S.[34]

In 1939 a German family asked Adolf Hitler to have their disabled son killed, the five-month-old boy was given a lethal dose of Luminal after Hitler sent his own doctor to examine him. A few days later 15 psychiatrists were summoned to Hitler's Chancellery and directed to commence a clandestine euthanasia program.[35][36] In 1940, at a clinic in Ansbach, Germany, around 50 intellectually disabled children were injected with Luminal and killed that way. A plaque was erected in their memory in 1988 in the local hospital at Feuchtwanger Strasse 38, although a newer plaque does not mention that patients were actively killed with barbiturates on site.[37][38] Luminal was used in the Nazi "children's euthanasia" program through at least 1943.[39] see also:[40]

Phenobarbital was used to treat neonatal jaundice by increasing liver metabolism and thus lowering bilirubin levels. In the 1950s, phototherapy was discovered, and became the standard treatment.[41]

Phenobarbital was used for over 25 years as prophylaxis in the treatment of febrile seizures.[42] Although an effective treatment in preventing recurrent febrile seizures, it had no positive effect on patient outcome or risk of developing epilepsy. The treatment of simple febrile seizures with anticonvulsant prophylaxis is no longer recommended.[43][44]

Names

Phenobarbital is the INN and phenobarbitone is the BAN.

Synthesis

Barbiturate drugs are obtained via condensation reactions between a derivative of diethyl malonate and urea in the presence of a strong base.[45] The synthesis of Phenobarbital uses this common approach as well but differs in the way in which this malonate derivative is obtained. The reason for this difference is due to the fact that aryl halides do not typically act as nucleophiles in Malonic ester synthesis in the same way as aliphatic organosulfates or halocarbons do.[46] To overcome this lack of chemical reactivity two dominant synthetic approaches using benzyl cyanide as a starting material have been developed:

The first of these methods consists of a Pinner reaction of benzyl cyanide, giving phenylacetic acid ethyl ester.[47] Subsequently this ester undergoes cross Claisen condensation using diethyl oxalate, giving diethyl ester of phenyloxobutandioic acid. Upon heating this intermediate easily loses carbon monoxide, yielding diethyl phenylmalonate.[48] Malonic ester synthesis using ethyl bromide leads to the formation of α-phenyl-α-ethylmalonic ester. Finally a condensation reaction with urea gives phenobarbital.[45]

The second approach utilizes diethyl carbonate in the presence of a strong base to give α-phenylcyanoacetic ester.[49][50] Alkylation of this ester using ethyl bromide proceeds via a nitrile anion intermediate to give the α-phenyl-α-ethylcyanoacetic ester.[51] This product is then further converted into the 4-iminoderivative upon condensation with urea. Finally acidic hydrolysis of the resulting product gives phenobarbital.[52]

Regulation

The level of regulation includes Schedule IV Non-Narcotic (depressant) (ACSCN 2285) in the United States under the Controlled Substances Act 1970—but along with a few other barbiturates and at least one benzodiazepine, and codeine, dionine, or dihydrocodeine at low concentrations, it also has exempt prescription and had at least one exempt OTC combination drug now more tightly regulated for its ephedrine content.[53] The phenobarbitone/phenobarbital exists in subtherapeutic doses which add up to an effective dose to counter the overstimulation and possible seizures from a deliberate overdose in ephedrine tablets for asthma, which are now regulated at the federal and state level as: a restricted OTC medicine and/or watched precursor, uncontrolled but watched/restricted prescription drug & watched precursor, a Schedule II, III, IV, or V prescription-only controlled substance & watched precursor, or a Schedule V (which also has possible regulations at the county/parish, town, city, or district as well aside from the fact that the pharmacist can also choose not to sell it, and photo ID and signing a register is required) exempt Non-Narcotic restricted/watched OTC medicine.[54]

References

  1. 1 2 3 4 5 6 7 8 9 10 11 "Phenobarbital". The American Society of Health-System Pharmacists. Retrieved Aug 14, 2015.
  2. 1 2 Marx, John A. (2010). Rosen's emergency medicine : concepts and clinical practice (7 ed.). Philadelphia: Mosby/Elsevier. p. 1352. ISBN 978-0-323-05472-0.
  3. 1 2 Ilangaratne, NB; Mannakkara, NN; Bell, GS; Sander, JW (Dec 1, 2012). "Phenobarbital: missing in action.". Bulletin of the World Health Organization 90 (12): 871–871A. doi:10.2471/BLT.12.113183. PMID 23284189.
  4. ↑ Brodie, MJ; Kwan, P (December 2012). "Current position of phenobarbital in epilepsy and its future.". Epilepsia. 53 Suppl 8: 40–6. doi:10.1111/epi.12027. PMID 23205961.
  5. ↑ "The epilepsies: the diagnosis and management of the epilepsies in adults and children in primary and secondary care NICE guidelines [CG137]". National Institute for Health and Care Excellence. January 2012.
  6. ↑ "Prescribing medicines in pregnancy database". Australian Government. 3 March 2014. Retrieved 22 April 2014.
  7. ↑ "Phenobarbital use while Breastfeeding". 2013. Retrieved 14 August 2015.
  8. ↑ Stevens, George M. Brenner, Craig W. (2013). Pharmacology (4th ed.). Philadelphia, PA: Elsevier/Saunders. p. 204. ISBN 978-1-4557-0278-7.
  9. ↑ Engel, Jerome (2008). Epilepsy : a comprehensive textbook (2nd ed.). Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. p. 1431. ISBN 978-0-7817-5777-5.
  10. ↑ "WHO Model List of Essential Medicines" (PDF). World Health Organization. March 2005. Retrieved 2006-03-12.
  11. 1 2 Newton, CR (29 September 2012). "Epilepsy in poor regions of the world.". Lancet 380 (9848): 1193–201. doi:10.1016/S0140-6736(12)61381-6. PMID 23021288.
  12. ↑ NICE (2005-10-27). "CG20 Epilepsy in adults and children: NICE guideline". NHS. Retrieved 2006-09-06.
  13. 1 2 British National Formulary 51
  14. ↑ Taylor S, Tudur Smith C, Williamson PR, Marson AG (2003). Taylor, Stephen, ed. "Phenobarbitone versus phenytoin monotherapy for partial onset seizures and generalized onset tonic-clonic seizures". Cochrane Database of Systematic Reviews (2): CD002217. doi:10.1002/14651858.CD002217. PMC 4176628. PMID 11687150. Retrieved 2006-09-06.
  15. ↑ Tudur Smith C, Marson AG, Williamson PR (2003). Tudur Smith, Catrin, ed. "Carbamazepine versus phenobarbitone monotherapy for epilepsy". Cochrane Database of Systematic Reviews (1): CD001904. doi:10.1002/14651858.CD001904. PMID 12535420. Retrieved 2006-09-06.
  16. ↑ Moshé, edited by Simon Shorvon, Emilio Perucca, Jerome Engel Jr. ; foreword by Solomon (2009). The treatment of epilepsy (3rd ed.). Chichester, UK: Wiley-Blackwell. p. 587. ISBN 978-1-4443-1667-4.
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  20. ↑ John M. Pellock, W. Edwin Dodson, Blaise F. D. Bourgeois (2001-01-01). Pediatric Epilepsy. Demos Medical Publishing. p. 152. ISBN 1-888799-30-7.
  21. ↑ Raj D Sheth (2005-03-30). "Neonatal Seizures". eMedicine. WebMD. Retrieved 2006-09-06.
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  23. ↑ http://www.aafp.org/afp/980700ap/miller.html
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  25. 1 2 Rania Habal (2006-01-27). "Barbiturate Toxicity". eMedicine. WebMD. Retrieved 2006-09-14.
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  27. ↑ King, Wayne (April 19, 1989). "Abbie Hoffman Committed Suicide Using Barbiturates, Autopsy Shows". The New York Times. Retrieved 2008-04-09.
  28. ↑ "Heaven's Gate cult members found dead". History Channel. Retrieved September 17, 2014.
  29. ↑ Thomas, WB (2003). "Seizures and narcolepsy". In Dewey, Curtis W. (ed.). A Practical Guide to Canine and Feline Neurology. Ames, Iowa: Iowa State Press. ISBN 0-8138-1249-6.
  30. ↑ Dodman, Nicholas. "Feline Hyperesthesia (FHS)". PetPlace.com. Retrieved 2011-11-17.
  31. ↑ Editor, Cynthia M. Kahn; associate editor Scott Line (February 8, 2005). Kahn, Cynthia M., Line, Scott, Aiello, Susan E. (ed.), ed. The Merck Veterinary Manual (9th ed.). John Wiley & Sons. ISBN 0-911910-50-6.
  32. ↑ Sneader, Walter (2005-06-23). Drug Discovery. John Wiley and Sons. p. 369. ISBN 0-471-89979-8.
  33. ↑ Ole Daniel Enersen. "Alfred Hauptmann". Retrieved 2006-09-06.
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  35. ↑ Zoech, Irene (12 October 2003). "Named: the baby boy who was Nazis' first euthanasia victim". The Telegraph. Retrieved 1 November 2013. The case was to provide the rationale for a secret Nazi decree that led to 'mercy killings' of almost 300,000 mentally and physically handicapped people. The Kretschmars wanted their son dead but most of the other children were forcibly taken from their parents to be killed.
  36. ↑ Wesley J. Smith (26 March 2006). "Killing Babies, Compassionately". Weekly Standard. Retrieved 1 November 2013. Hitler later signed a secret decree permitting the euthanasia of disabled infants. Sympathetic physicians and nurses from around the country--many not even Nazi party members--cooperated in the horror that followed. Formal 'protective guidelines' were created, including the creation of a panel of 'expert referees,' which judged which infants were eligible for the program.
  37. ↑ Kaelber, Lutz (8 March 2013). "Kinderfachabteilung Ansbach". Sites of Nazi "Children's 'Euthanasia'" Crimes and Their Commemoration in Europe. University of Vermont. Retrieved 1 November 2013. In the late 1980s, important developments occurred at the clinic that led to the first publication on the subject and the display of two plaques. Dr. Reiner Weisenseel wrote his dissertation under Dr. Athen, then the director of the Ansbacher Bezirkskrankenhaus, on the involvement of the clinic in Euthanasia crimes, including the operation of the Kinderfachabteilung. In 1988 two members of the Green Party as well as the regional diet (Bezirkstag) were horrified to find portraits of physicians involved in Nazi euthanasia crimes among the honorary display of medical personnel in the administrative building, and they successfully petitioned to have these portraits removed. Since 1992 a plaque hangs in the entry hall way of the administrative building. It reads: 'In the Third Reich the Ansbach facility delivered to their death more than 2000 of the patients entrusted to it as life unworthy of living: They were transferred to killing facilities or starved to death. In their own way many people incurred responsibility.' It continues: 'Half a century later full of shame we commemorate the victims and call to remember the Fifth Commandment.' The active killing of children specifically transferred to the clinic to be murdered is not noted. The plaque does not address that that euthanasia victims were not only starved or transported to gassing facilities but actively killed with barbiturates on site.
  38. ↑ Binder, Johann (October 2011). "Die Heil- und Pflegeanstalt Ansbach während des Nationalsozialismus" (PDF). Bezirksklinikum Ansbach (in German). Bezirkskliniken Mittelfranken. Retrieved 1 November 2013.
  39. ↑ Kaelber, Lutz (Spring 2013). "Jewish Children with Disabilities and Nazi "Euthanasia" Crimes" (PDF). The Bulletin of the Carolyn and Leonard Miller Center for Holocaust Studies. University of Vermont. Retrieved 1 November 2013. Two Polish physicians reported at the time that 235 children from ages up to 14 were listed in the booklet, of whom 221 had died. An investigation revealed that the medical records of the children had been falsified, as those records showed a far lower dosage of Luminal given to them than was entered into the Luminal booklet. For example, the medical records for Marianna N. showed for 16 January 1943 (she died on that day) a dosage of .1 gram of Luminal, whereas the Luminal booklet showed the actual dosage as .4 grams, or four times the dosage recommended for her body weight.
  40. ↑ López-Muñoz, Francisco; Alamo, Cecilio; García-García, Pilar; Molina, Juan D.; Rubio, Gabriel (2008). "The role of psychopharmacology in the medical abuses of the Third Reich: From euthanasia programmes to human experimentation". Brain Research Bulletin 77 (6): 388–403. doi:10.1016/j.brainresbull.2008.09.002. PMID 18848972.
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  42. ↑ John M. Pellock, W. Edwin Dodson, Blaise F. D. Bourgeois (2001-01-01). Pediatric Epilepsy. Demos Medical Publishing. p. 169. ISBN 1-888799-30-7.
  43. ↑ Robert Baumann (2005-02-14). "Febrile Seizures". eMedicine. WebMD. Retrieved 2006-09-06.
  44. ↑ various (March 2005). "Diagnosis and management of epilepsies in children and young people" (PDF). Scottish Intercollegiate Guidelines Network. p. 15. Retrieved 2006-09-07.
  45. 1 2 Furniss, Brian; Hannaford, Antony; Smith, Peter; and Tatchell, Austin (1996). Vogel's Textbook of Practical Organic Chemistry 5th Ed. London: Longman Science & Technical. pp. 1174–1179. ISBN 978-0-582-46236-6.
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  47. ↑ Adams, Roger; Thal, A. F. (1922). "Ethyl Phenylacetate". Organic Syntheses 2: 27. doi:10.15227/orgsyn.002.0027.
  48. ↑ Meyer, G. M.; Levene, P. A. (1936). "Diethyl phenylmalonate". Organic Syntheses 16: 33. doi:10.15227/orgsyn.016.0033.
  49. ↑ Chamberlain, J. S.; Chap, J.J.; Doyle, J. E.; Spaulding, L. B. (1935). "The Synthesis of 5,5-Alkylphenylbarbituric Acids". Journal of the American Chemical Society 57 (2): 352–354. doi:10.1021/ja01305a036.
  50. ↑ Nelson, William L.; Cretcher, Leonard H. (1928). "The Preparation of Ethyl Phenylmalonate and of 5-Phenyl-beta-hydroxyethylbarbituric acid". Journal of the American Chemical Society 50 (10): 2758–2762. doi:10.1021/ja01397a029.
  51. ↑ Makosza, M.; Jonczyk, A (1976). "Phase-Transfer Alkylation of Nitriles: 2-Phenylbutyronitrile". Organic Synthesis 55: 91. doi:10.15227/orgsyn.055.0091.
  52. ↑ Marc, Inman Nyack; Haverstraw, N.; Bilter, William. "Preparation of Phenobarbital (US2358072, 1944)" (PDF). Google Patents. Kay-Fries Chemicals, Inc. Retrieved 30 October 2014.
  53. ↑ http://www.deadiversion.usdoj.gov/schedules/orangebook/c_cs_alpha.pdf pp 12 printed PDF 23.I.2016
  54. ↑ http://www.deadiversion.usdoj.gov/schedules/orangebook/c_cs_alpha.pdf page 1,7, 12, accessed 23.I.2016

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