MAST2

Microtubule associated serine/threonine kinase 2
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2KQF​, 2KYL​
Identifiers
Symbols	MAST2 ; MAST205; MTSSK
External IDs	OMIM: 612257 MGI: 894676 HomoloGene: 7428 IUPHAR: 1511 GeneCards: MAST2 Gene
EC number	2.7.11.1
Gene ontology Molecular function • magnesium ion binding • protein serine/threonine kinase activity • protein binding • ATP binding • microtubule binding • phosphatase binding Cellular component • cytoplasm • plasma membrane • microtubule cytoskeleton Biological process • protein phosphorylation • cytoskeleton organization • peptidyl-serine phosphorylation • intracellular signal transduction • regulation of interleukin-12 biosynthetic process • spermatid differentiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23139	17776
Ensembl	ENSG00000086015	ENSMUSG00000003810
UniProt	Q6P0Q8	Q60592
RefSeq (mRNA)	NM_015112	NM_001042743
RefSeq (protein)	NP_055927	NP_001036208
Location (UCSC)	Chr 1: 45.79 – 46.04 Mb	Chr 4: 116.31 – 116.46 Mb
PubMed search

Microtubule-associated serine/threonine-protein kinase 2 is an enzyme that in humans is encoded by the MAST2 gene.^[1] The protein encoded by this gene controls TRAF6 and NF-kappaB activity.^[2]

Interactions

MAST2 has been shown to interact with PCLKC.^[3]

Model organisms

Model organisms have been used in the study of MAST2 function. A conditional knockout mouse line called Mast2^{tm1a(KOMP)Wtsi} has been generated.^[4] Male and female animals underwent a standardized phenotypic screen^[5] to determine the effects of deletion.^[6][7][8][9] Additional screens performed: - In-depth immunological phenotyping^[10]

Mast2 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[5][10]{
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Influenza Challenge	Normal

References

1. "Entrez Gene: MAST2 microtubule associated serine/threonine kinase 2".
2. Xiong H, Li H, Chen Y, Zhao J, Unkeless JC (2004). "Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability". J. Biol. Chem. 279 (42): 43675–83. doi:10.1074/jbc.M404328200. PMID 15308666.
3. Okazaki N, Takahashi N, Kojima S, Masuho Y, Koga H (July 2002). "Protocadherin LKC, a new candidate for a tumor suppressor of colon and liver cancers, its association with contact inhibition of cell proliferation". Carcinogenesis 23 (7): 1139–48. doi:10.1093/carcin/23.7.1139. PMID 12117771.
4. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
5. "International Mouse Phenotyping Consortium".
6. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
7. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
8. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
9. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
10. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Walden PD, Cowan NJ (1993). "A novel 205-kilodalton testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette". Mol. Cell. Biol. 13 (12): 7625–35. doi:10.1128/mcb.13.12.7625. PMC 364834. PMID 8246979.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Walden PD, Millette CF (1996). "Increased activity associated with the MAST205 protein kinase complex during mammalian spermiogenesis". Biol. Reprod. 55 (5): 1039–44. doi:10.1095/biolreprod55.5.1039. PMID 8902215.
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (5): 277–86. doi:10.1093/dnares/5.5.277. PMID 9872452.
• Lumeng C, Phelps S, Crawford GE, Walden PD, Barald K, Chamberlain JS (1999). "Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases". Nat. Neurosci. 2 (7): 611–7. doi:10.1038/10165. PMID 10404183.
• Adey NB, Huang L, Ormonde PA, Baumgard ML, Pero R, Byreddy DV, Tavtigian SV, Bartel PL (2000). "Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and stimulates PDZ binding". Cancer Res. 60 (1): 35–7. PMID 10646847.
• Gisler SM, Stagljar I, Traebert M, Bacic D, Biber J, Murer H (2001). "Interaction of the type IIa Na/Pi cotransporter with PDZ proteins". J. Biol. Chem. 276 (12): 9206–13. doi:10.1074/jbc.M008745200. PMID 11099500.
• Okazaki N, Takahashi N, Kojima S, Masuho Y, Koga H (2002). "Protocadherin LKC, a new candidate for a tumor suppressor of colon and liver cancers, its association with contact inhibition of cell proliferation". Carcinogenesis 23 (7): 1139–48. doi:10.1093/carcin/23.7.1139. PMID 12117771.
• Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Navarro-Lérida I, Martínez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodríguez-Crespo I (2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics 4 (2): 339–46. doi:10.1002/pmic.200300528. PMID 14760703.
• Zhou H, Xiong H, Li H, Plevy SE, Walden PD, Sassaroli M, Prestwich GD, Unkeless JC (2004). "Microtubule-associated serine/threonine kinase-205 kDa and Fc gamma receptor control IL-12 p40 synthesis and NF-kappa B activation". J. Immunol. 172 (4): 2559–68. doi:10.4049/jimmunol.172.4.2559. PMID 14764729.
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Xiong H, Li H, Chen Y, Zhao J, Unkeless JC (2004). "Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability". J. Biol. Chem. 279 (42): 43675–83. doi:10.1074/jbc.M404328200. PMID 15308666.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.