MYLK

Myosin light chain kinase
PDB rendering based on 2cqv.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2CQV​, 2K0F​, 2YR3​
Identifiers
Symbols	MYLK ; AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK1; smMLCK
External IDs	OMIM: 600922 MGI: 894806 HomoloGene: 14202 ChEMBL: 2428 GeneCards: MYLK Gene
EC number	2.7.11.18
Gene ontology Molecular function • actin binding • calmodulin-dependent protein kinase activity • myosin light chain kinase activity • protein binding • calmodulin binding • ATP binding • metal ion binding Cellular component • stress fiber • cytoplasm • cytosol • cell-cell junction • lamellipodium • cleavage furrow • extracellular exosome Biological process • positive regulation of defense response to virus by host • protein phosphorylation • muscle contraction • smooth muscle contraction • actin filament organization • small GTPase mediated signal transduction • tonic smooth muscle contraction • positive regulation of cell migration • bleb assembly • positive regulation of calcium ion transport • aorta smooth muscle tissue morphogenesis • cellular hypotonic response • positive regulation of wound healing • activation of mitophagy in response to mitochondrial depolarization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4638	107589
Ensembl	ENSG00000065534	ENSMUSG00000022836
UniProt	Q15746	B1B1A8
RefSeq (mRNA)	NM_005965	NM_139300
RefSeq (protein)	NP_444253	NP_647461
Location (UCSC)	Chr 3: 123.61 – 123.88 Mb	Chr 16: 34.75 – 35 Mb
PubMed search

Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene.^[1]

Function

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.^[2]

See also

• Myosin light-chain kinase

References

1. Potier MC, Chelot E, Pekarsky Y, Gardiner K, Rossier J, Turnell WG (Mar 1996). "The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21". Genomics 29 (3): 562–70. doi:10.1006/geno.1995.9965. PMID 8575746.
2. "Entrez Gene: MYLK myosin, light chain kinase".

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Clayburgh DR, Rosen S, Witkowski ED, et al. (2005). "A Differentiation-dependent Splice Variant of Myosin Light Chain Kinase, MLCK1, Regulates Epithelial Tight Junction Permeability". J. Biol. Chem. 279 (53): 55506–13. doi:10.1074/jbc.M408822200. PMC 1237105. PMID 15507455.
• Wang F, Graham WV, Wang Y, et al. (2005). "Interferon-γ and Tumor Necrosis Factor-α Synergize to Induce Intestinal Epithelial Barrier Dysfunction by Up-Regulating Myosin Light Chain Kinase Expression". Am. J. Pathol. 166 (2): 409–19. doi:10.1016/S0002-9440(10)62264-X. PMC 1237049. PMID 15681825.
• Russo JM, Florian P, Shen L, et al. (2005). "Distinct Temporal-Spatial Roles for Rho Kinase and Myosin Light Chain Kinase in Epithelial Purse-String Wound Closure". Gastroenterology 128 (4): 987–1001. doi:10.1053/j.gastro.2005.01.004. PMC 1237051. PMID 15825080.
• Shimizu S, Yoshida T, Wakamori M, et al. (2006). "Ca2+–calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells". J. Physiol. (Lond.) 570 (Pt 2): 219–35. doi:10.1113/jphysiol.2005.097998. PMC 1464317. PMID 16284075.
• Kim MT, Kim BJ, Lee JH, et al. (2006). "Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells". Am. J. Physiol., Cell Physiol. 290 (4): C1031–40. doi:10.1152/ajpcell.00602.2004. PMID 16306123.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell. Sci. 119 (Pt 11): 2269–81. doi:10.1242/jcs.02926. PMID 16723733.
• Seguchi O, Takashima S, Yamazaki S, et al. (2007). "A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart". J. Clin. Invest. 117 (10): 2812–24. doi:10.1172/JCI30804. PMC 1978424. PMID 17885681.

External links

• MYLK protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.