PRKCQ

Protein kinase C, theta
PDB rendering based on 1xjd.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1XJD​, 2ENJ​, 2ENN​, 2ENZ​, 2JED​, 4Q9Z​, 4RA5​
Identifiers
Symbols	PRKCQ ; PRKCT; nPKC-theta
External IDs	OMIM: 600448 MGI: 97601 HomoloGene: 21263 ChEMBL: 3920 GeneCards: PRKCQ Gene
EC number	2.7.11.13
Gene ontology Molecular function • protein serine/threonine kinase activity • protein kinase C activity • ubiquitin-protein transferase activity • protein binding • ATP binding • metal ion binding Cellular component • immunological synapse • nucleus • cytosol • plasma membrane • neuromuscular junction • sarcolemma Biological process • regulation of cell growth • response to hypoxia • regulation of transcription, DNA-templated • protein phosphorylation • membrane protein ectodomain proteolysis • apoptotic process • cellular component disassembly involved in execution phase of apoptosis • inflammatory response • axon guidance • aging • blood coagulation • phototransduction, visible light • response to heat • response to glucose • regulation of G2/M transition of mitotic cell cycle • programmed cell death • response to organic cyclic compound • rhodopsin mediated signaling pathway • protein ubiquitination • regulation of vasoconstriction • regulation of rhodopsin mediated signaling pathway • platelet activation • positive regulation of interleukin-17 production • positive regulation of interleukin-4 production • response to insulin • intracellular signal transduction • Fc-epsilon receptor signaling pathway • positive regulation of T cell proliferation • tissue regeneration • positive regulation of NF-kappaB import into nucleus • positive regulation of interleukin-2 biosynthetic process • innate immune response • respiratory burst • negative regulation of insulin receptor signaling pathway • positive regulation of protein secretion • T cell receptor signaling pathway • positive regulation of T cell activation • positive regulation of NF-kappaB transcription factor activity • positive regulation of filopodium assembly • positive regulation of stress fiber assembly • cell chemotaxis • negative regulation of T cell apoptotic process • regulation of platelet aggregation • positive regulation of T-helper 17 type immune response • positive regulation of T-helper 2 cell activation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5588	18761
Ensembl	ENSG00000065675	ENSMUSG00000026778
UniProt	Q04759	Q02111
RefSeq (mRNA)	NM_001242413	NM_008859
RefSeq (protein)	NP_001229342	NP_032885
Location (UCSC)	Chr 10: 6.43 – 6.58 Mb	Chr 2: 11.17 – 11.3 Mb
PubMed search

Protein kinase C theta (PKC-θ) is an enzyme that in humans is encoded by the PRKCQ gene.^[1]

Function

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.^[2]

Interactions

PRKCQ has been shown to interact with:

• AKT1^[3]
• FYN,^[4]
• GLRX3,^[5] and
• VAV1.^[6]

PRKCQ has been shown to phosphorylate CARD11 as part of the NF-κB signaling pathway.^[7]

Inhibitors

• (R)-2-((S)-4-(3-Chloro-5-fluoro-6-(1H-pyrazolo[3,4-b]pyridin- 3-yl)pyridin-2-yl)piperazin-2-yl)-3-methylbutan-2-ol^[8]

See also

• Protein kinase C

References

1. Baier G, Telford D, Giampa L, Coggeshall KM, Baier-Bitterlich G, Isakov N, Altman A (April 1993). "Molecular cloning and characterization of PKC theta, a novel member of the protein kinase C (PKC) gene family expressed predominantly in hematopoietic cells". J Biol Chem 268 (7): 4997–5004. PMID 8444877.
2. "Entrez Gene: PRKCQ protein kinase C, theta".
3. Bauer B, Krumböck N, Fresser F, Hochholdinger F, Spitaler M, Simm A, Uberall F, Schraven B, Baier G (August 2001). "Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells". J. Biol. Chem. 276 (34): 31627–34. doi:10.1074/jbc.M103098200. PMID 11410591.
4. Ron D, Napolitano EW, Voronova A, Vasquez NJ, Roberts DN, Calio BL, Caothien RH, Pettiford SM, Wellik S, Mandac JB, Kauvar LM (July 1999). "Direct interaction in T-cells between thetaPKC and the tyrosine kinase p59fyn". J. Biol. Chem. 274 (27): 19003–10. doi:10.1074/jbc.274.27.19003. PMID 10383400.
5. Witte S, Villalba M, Bi K, Liu Y, Isakov N, Altman A (January 2000). "Inhibition of the c-Jun N-terminal kinase/AP-1 and NF-kappaB pathways by PICOT, a novel protein kinase C-interacting protein with a thioredoxin homology domain". J. Biol. Chem. 275 (3): 1902–9. doi:10.1074/jbc.275.3.1902. PMID 10636891.
6. Hehner SP, Li-Weber M, Giaisi M, Dröge W, Krammer PH, Schmitz ML (April 2000). "Vav synergizes with protein kinase C theta to mediate IL-4 gene expression in response to CD28 costimulation in T cells". J. Immunol. 164 (7): 3829–36. doi:10.4049/jimmunol.164.7.3829. PMID 10725744.
7. Takeda K, Harada Y, Watanabe R, Inutake Y, Ogawa S, Onuki K, Kagaya S, Tanabe K, Kishimoto H, Abe R (December 2008). "CD28 stimulation triggers NF-kappaB activation through the CARMA1-PKCtheta-Grb2/Gads axis.". Int. Immunol. 20 (12): 1507–15. doi:10.1093/intimm/dxn108. PMID 18829987.
8. Jimenez JM, Boyall D, Brenchley G, Collier PN, Davis CJ, Fraysse D, Keily SB, Henderson J, Miller A, Pierard F, Settimo L, Twin HC, Bolton CM, Curnock AP, Chiu P, Tanner AJ, Young S (2013). "Design and optimization of selective protein kinase C θ (PKCθ) inhibitors for the treatment of autoimmune diseases". J. Med. Chem. 56 (5): 1799–810. doi:10.1021/jm301465a. PMID 23398373.

Further reading

• Meller N, Altman A, Isakov N (1998). "New perspectives on PKCtheta, a member of the novel subfamily of protein kinase C.". Stem Cells 16 (3): 178–92. doi:10.1002/stem.160178. PMID 9617893.
• Greenway AL, Holloway G, McPhee DA, Ellis P, Cornall A, Lidman M (2004). "HIV-1 Nef control of cell signalling molecules: multiple strategies to promote virus replication.". J. Biosci. 28 (3): 323–35. doi:10.1007/BF02970151. PMID 12734410.
• Ruegg CL, Strand M (1991). "A synthetic peptide with sequence identity to the transmembrane protein GP41 of HIV-1 inhibits distinct lymphocyte activation pathways dependent on protein kinase C and intracellular calcium influx.". Cell. Immunol. 137 (1): 1–13. doi:10.1016/0008-8749(91)90051-C. PMID 1832084.
• Chowdhury IH, Koyanagi Y, Kobayashi S, Hamamoto Y, Yoshiyama H, Yoshida T, Yamamoto N (1990). "The phorbol ester TPA strongly inhibits HIV-1-induced syncytia formation but enhances virus production: possible involvement of protein kinase C pathway.". Virology 176 (1): 126–32. doi:10.1016/0042-6822(90)90237-L. PMID 1970444.
• Ruegg CL, Strand M (1990). "Inhibition of protein kinase C and anti-CD3-induced Ca2+ influx in Jurkat T cells by a synthetic peptide with sequence identity to HIV-1 gp41.". J. Immunol. 144 (10): 3928–35. PMID 2139676.
• Jakobovits A, Rosenthal A, Capon DJ (1990). "Trans-activation of HIV-1 LTR-directed gene expression by tat requires protein kinase C.". EMBO J. 9 (4): 1165–70. PMC 551792. PMID 2182321.
• Fields AP, Bednarik DP, Hess A, May WS (1988). "Human immunodeficiency virus induces phosphorylation of its cell surface receptor.". Nature 333 (6170): 278–80. doi:10.1038/333278a0. PMID 3259291.
• Chirmule N, Goonewardena H, Pahwa S, Pasieka R, Kalyanaraman VS, Pahwa S (1995). "HIV-1 envelope glycoproteins induce activation of activated protein-1 in CD4+ T cells.". J. Biol. Chem. 270 (33): 19364–9. doi:10.1074/jbc.270.33.19364. PMID 7642615.
• Chang JD, Xu Y, Raychowdhury MK, Ware JA (1993). "Molecular cloning and expression of a cDNA encoding a novel isoenzyme of protein kinase C (nPKC). A new member of the nPKC family expressed in skeletal muscle, megakaryoblastic cells, and platelets.". J. Biol. Chem. 268 (19): 14208–14. PMID 7686153.
• Erdel M, Baier-Bitterlich G, Duba C, Isakov N, Altman A, Utermann G, Baier G (1995). "Mapping of the human protein kinase C-theta (PRKCQ) gene locus to the short arm of chromosome 10 (10p15) by FISH.". Genomics 25 (2): 595–7. doi:10.1016/0888-7543(95)80068-W. PMID 7790001.
• Ward NE, Gravitt KR, O'Brian CA (1995). "Inhibition of protein kinase C by a synthetic peptide corresponding to cytoplasmic domain residues 828-848 of the human immunodeficiency virus type 1 envelope glycoprotein.". Cancer Lett. 88 (1): 37–40. doi:10.1016/0304-3835(94)03610-U. PMID 7850771.
• Gupta S, Aggarwal S, Kim C, Gollapudi S (1994). "Human immunodeficiency virus-1 recombinant gp120 induces changes in protein kinase C isozymes--a preliminary report.". Int. J. Immunopharmacol. 16 (3): 197–204. doi:10.1016/0192-0561(94)90013-2. PMID 8206685.
• Parada NA, Cruikshank WW, Danis HL, Ryan TC, Center DM (1996). "IL-16- and other CD4 ligand-induced migration is dependent upon protein kinase C.". Cell. Immunol. 168 (1): 100–6. doi:10.1006/cimm.1996.0054. PMID 8599832.
• Conant K, Ma M, Nath A, Major EO (1996). "Extracellular human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kappa B binding and protein kinase C activity in primary human astrocytes.". J. Virol. 70 (3): 1384–9. PMC 189957. PMID 8627654.
• Smith BL, Krushelnycky BW, Mochly-Rosen D, Berg P (1996). "The HIV nef protein associates with protein kinase C theta.". J. Biol. Chem. 271 (28): 16753–7. doi:10.1074/jbc.271.17.9906. PMID 8663223.
• Meller N, Liu YC, Collins TL, Bonnefoy-Bérard N, Baier G, Isakov N, Altman A (1996). "Direct interaction between protein kinase C theta (PKC theta) and 14-3-3 tau in T cells: 14-3-3 overexpression results in inhibition of PKC theta translocation and function.". Mol. Cell. Biol. 16 (10): 5782–91. PMC 231579. PMID 8816492.
• Holmes AM (1996). "In vitro phosphorylation of human immunodeficiency virus type 1 Tat protein by protein kinase C: evidence for the phosphorylation of amino acid residue serine-46.". Arch. Biochem. Biophys. 335 (1): 8–12. doi:10.1006/abbi.1996.0476. PMID 8914829.
• Monks CR, Kupfer H, Tamir I, Barlow A, Kupfer A (1997). "Selective modulation of protein kinase C-theta during T-cell activation.". Nature 385 (6611): 83–6. doi:10.1038/385083a0. PMID 8985252.
• Datta R, Kojima H, Yoshida K, Kufe D (1997). "Caspase-3-mediated cleavage of protein kinase C theta in induction of apoptosis.". J. Biol. Chem. 272 (33): 20317–20. doi:10.1074/jbc.272.33.20317. PMID 9252332.