SMG1 (gene)

SMG1 phosphatidylinositol 3-kinase-related kinase
Identifiers
Symbols	SMG1 ; 61E3.4; ATX; LIP
External IDs	OMIM: 607032 MGI: 1919742 HomoloGene: 56697 ChEMBL: 1795195 GeneCards: SMG1 Gene
EC number	2.7.11.1
Gene ontology Molecular function • protein kinase activity • protein serine/threonine kinase activity • protein binding • ATP binding • poly(A) RNA binding • metal ion binding • protein dimerization activity Cellular component • nucleus • cytoplasm • cytosol Biological process • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • double-strand break repair via homologous recombination • mRNA export from nucleus • response to stress • gene expression • peptidyl-serine phosphorylation • protein autophosphorylation • phosphatidylinositol phosphorylation Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23049	233789
Ensembl	ENSG00000157106	ENSMUSG00000030655
UniProt	Q96Q15	Q8BKX6
RefSeq (mRNA)	NM_015092	NM_001031814
RefSeq (protein)	NP_055907	NP_001026984
Location (UCSC)	Chr 16: 18.8 – 18.93 Mb	Chr 7: 118.13 – 118.24 Mb
PubMed search

Serine/threonine-protein kinase SMG1 is an enzyme that in humans is encoded by the SMG1 gene.^[1][2][3][4] SMG1 belongs to the phosphatidylinositol 3-kinase-related kinase protein family.

Function

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.^[4]

Interactions

SMG1 (gene) has been shown to interact with PRKCI^[5] and UPF1.^[6]

References

1. Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477.
2. Denning G, Jamieson L, Maquat LE, Thompson EA, Fields AP (June 2001). "Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein". J. Biol. Chem. 276 (25): 22709–14. doi:10.1074/jbc.C100144200. PMID 11331269.
3. Morita T, Yamashita A, Kashima I, Ogata K, Ishiura S, Ohno S (March 2007). "Distant N- and C-terminal domains are required for intrinsic kinase activity of SMG-1, a critical component of nonsense-mediated mRNA decay". J. Biol. Chem. 282 (11): 7799–808. doi:10.1074/jbc.M610159200. PMID 17229728.
4. "Entrez Gene: SMG1 PI-3-kinase-related kinase SMG-1".
5. Diaz-Meco MT, Municio MM, Sanchez P, Lozano J, Moscat J (January 1996). "Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo". Mol. Cell. Biol. 16 (1): 105–14. PMC 230983. PMID 8524286.
6. Yamashita A, Ohnishi T, Kashima I, Taya Y, Ohno S (September 2001). "Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay". Genes Dev. 15 (17): 2215–28. doi:10.1101/gad.913001. PMC 312771. PMID 11544179.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Abraham RT (2005). "The ATM-related kinase, hSMG-1, bridges genome and RNA surveillance pathways". DNA Repair (Amst.) 3 (8-9): 919–25. doi:10.1016/j.dnarep.2004.04.003. PMID 15279777.
• Diaz-Meco MT, Municio MM, Sanchez P, Lozano J, Moscat J (1996). "Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo". Mol. Cell. Biol. 16 (1): 105–14. PMC 230983. PMID 8524286.
• Sanchez P, De Carcer G, Sandoval IV, Moscat J, Diaz-Meco MT (1998). "Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62". Mol. Cell. Biol. 18 (5): 3069–80. PMC 110686. PMID 9566925.
• Carmeliet P, Lampugnani MG, Moons L, Breviario F, Compernolle V, Bono F, Balconi G, Spagnuolo R, Oosthuyse B, Dewerchin M, Zanetti A, Angellilo A, Mattot V, Nuyens D, Lutgens E, Clotman F, de Ruiter MC, Gittenberger-de Groot A, Poelmann R, Lupu F, Herbert JM, Collen D, Dejana E (1999). "Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis". Cell 98 (2): 147–57. doi:10.1016/S0092-8674(00)81010-7. PMID 10428027.
• Loftus BJ, Kim UJ, Sneddon VP, Kalush F, Brandon R, Fuhrmann J, Mason T, Crosby ML, Barnstead M, Cronin L, Deslattes Mays A, Cao Y, Xu RX, Kang HL, Mitchell S, Eichler EE, Harris PC, Venter JC, Adams MD (1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.
• Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, de Oliveira PS, Bucher P, Jongeneel CV, O'Hare MJ, Soares F, Brentani RR, Reis LF, de Souza SJ, Simpson AJ (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491–6. doi:10.1073/pnas.97.7.3491. PMC 16267. PMID 10737800.
• Yamashita A, Ohnishi T, Kashima I, Taya Y, Ohno S (2001). "Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay". Genes Dev. 15 (17): 2215–28. doi:10.1101/gad.913001. PMC 312771. PMID 11544179.
• Eichler EE, Johnson ME, Alkan C, Tuzun E, Sahinalp C, Misceo D, Archidiacono N, Rocchi M (2002). "Divergent origins and concerted expansion of two segmental duplications on chromosome 16". J. Hered. 92 (6): 462–8. doi:10.1093/jhered/92.6.462. PMID 11948212.
• Chiu SY, Serin G, Ohara O, Maquat LE (2003). "Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1". RNA 9 (1): 77–87. doi:10.1261/rna.2137903. PMC 1370372. PMID 12554878.
• Ohnishi T, Yamashita A, Kashima I, Schell T, Anders KR, Grimson A, Hachiya T, Hentze MW, Anderson P, Ohno S (2003). "Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7". Mol. Cell 12 (5): 1187–200. doi:10.1016/S1097-2765(03)00443-X. PMID 14636577.
• Brumbaugh KM, Otterness DM, Geisen C, Oliveira V, Brognard J, Li X, Lejeune F, Tibbetts RS, Maquat LE, Abraham RT (2004). "The mRNA surveillance protein hSMG-1 functions in genotoxic stress response pathways in mammalian cells". Mol. Cell 14 (5): 585–98. doi:10.1016/j.molcel.2004.05.005. PMID 15175154.
• Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S (2006). "Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay". Genes Dev. 20 (3): 355–67. doi:10.1101/gad.1389006. PMC 1361706. PMID 16452507.
• Azzalin CM, Lingner J (2006). "The human RNA surveillance factor UPF1 is required for S phase progression and genome stability". Curr. Biol. 16 (4): 433–9. doi:10.1016/j.cub.2006.01.018. PMID 16488880.