Keratin 7

Keratin 7, type II
Identifiers
Symbols	KRT7 ; CK7; K2C7; K7; SCL
External IDs	OMIM: 148059 MGI: 96704 HomoloGene: 4058 GeneCards: KRT7 Gene
Gene ontology Molecular function • structural molecule activity • protein binding Cellular component • nucleus • cytoplasm • intermediate filament • keratin filament • extracellular exosome Biological process • viral process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	3855	110310
Ensembl	ENSG00000135480	ENSMUSG00000023039
UniProt	P08729	Q9DCV7
RefSeq (mRNA)	NM_005556	NM_033073
RefSeq (protein)	NP_005547	NP_149064
Location (UCSC)	Chr 12: 52.23 – 52.25 Mb	Chr 15: 101.41 – 101.43 Mb
PubMed search

Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene.^[1][2][3] Keratin 7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Function

Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.^[3]

Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. It is commonly used together with CK20 when making such diagnoses.^[4]

Model organisms

Model organisms have been used in the study of KRT7 function. A conditional knockout mouse line called Krt7^{tm1b(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[5] Male and female animals underwent a standardized phenotypic screen^[6] to determine the effects of deletion.^{[7][8][9][10]} Additional screens performed: - In-depth immunological phenotyping^[11]

Krt7 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[6][11]
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. Rosenberg M, Fuchs E, Le Beau MM, Eddy RL, Shows TB (Aug 1991). "Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12". Cytogenetics and Cell Genetics 57 (1): 33–8. doi:10.1159/000133109. PMID 1713141.
2. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (Jul 2006). "New consensus nomenclature for mammalian keratins". The Journal of Cell Biology 174 (2): 169–74. doi:10.1083/jcb.200603161. PMC 2064177. PMID 16831889.
3. "Entrez Gene: KRT7 keratin 7".
4. Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2 ed.). Greenwich Medical Media, Ltd. p. 173. ISBN 1-84110-100-1.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. "International Mouse Phenotyping Consortium".
7. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
8. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
9. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
10. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
11. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Bouwens L (Mar 1998). "Cytokeratins and cell differentiation in the pancreas". The Journal of Pathology 184 (3): 234–9. doi:10.1002/(SICI)1096-9896(199803)184:3<234::AID-PATH28>3.0.CO;2-D. PMID 9614373.
• Glass C, Kim KH, Fuchs E (Dec 1985). "Sequence and expression of a human type II mesothelial keratin". The Journal of Cell Biology 101 (6): 2366–73. doi:10.1083/jcb.101.6.2366. PMC 2114014. PMID 2415537.
• Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P (May 1987). "Tissue distribution of keratin 7 as monitored by a monoclonal antibody". Experimental Cell Research 170 (1): 235–49. doi:10.1016/0014-4827(87)90133-9. PMID 2436934.
• Glass C, Fuchs E (Oct 1988). "Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells". The Journal of Cell Biology 107 (4): 1337–50. doi:10.1083/jcb.107.4.1337. PMC 2115265. PMID 2459129.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Gress TM, Müller-Pillasch F, Geng M, Zimmerhackl F, Zehetner G, Friess H, Büchler M, Adler G, Lehrach H (Oct 1996). "A pancreatic cancer-specific expression profile". Oncogene 13 (8): 1819–30. PMID 8895530.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Alvarez Alvarez C, Sánchez Merino JM, Busto Castañón L, Pombo Felipe F, Arnal Monreal F (Jun 1998). "[Mucinous adenocarcinoma of the urachus synchronic with colorectal adenocarcinoma. Value of immunohistochemistry in the differential diagnosis]". Actas Urologicas Españolas 22 (6): 515–8. PMID 9734130.
• Kaba A, Jiang PH, Chany-Fournier F, Chany C (Jul 1999). "Sarcolectin (SCL): structure and expression of the recombinant molecule". Biochimie 81 (7): 709–15. doi:10.1016/S0300-9084(99)80128-X. PMID 10492017.
• Lin L, Holbro T, Alonso G, Gerosa D, Burger MM (2001). "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7". Journal of Cellular Biochemistry 80 (4): 483–90. doi:10.1002/1097-4644(20010315)80:4<483::AID-JCB1002>3.0.CO;2-B. PMID 11169732.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Harris RA, Yang A, Stein RC, Lucy K, Brusten L, Herath A, Parekh R, Waterfield MD, O'Hare MJ, Neville MA, Page MJ, Zvelebil MJ (Feb 2002). "Cluster analysis of an extensive human breast cancer cell line protein expression map database". Proteomics 2 (2): 212–23. doi:10.1002/1615-9861(200202)2:2<212::AID-PROT212>3.0.CO;2-H. PMID 11840567.
• Bonvini P, Gastaldi T, Falini B, Rosolen A (Mar 2002). "Nucleophosmin-anaplastic lymphoma kinase (NPM-ALK), a novel Hsp90-client tyrosine kinase: down-regulation of NPM-ALK expression and tyrosine phosphorylation in ALK(+) CD30(+) lymphoma cells by the Hsp90 antagonist 17-allylamino,17-demethoxygeldanamycin". Cancer Research 62 (5): 1559–66. PMID 11888936.
• Jovanovic I, Tzardi M, Mouzas IA, Micev M, Pesko P, Milosavljevic T, Zois M, Sganzos M, Delides G, Kanavaros P (Apr 2002). "Changing pattern of cytokeratin 7 and 20 expression from normal epithelium to intestinal metaplasia of the gastric mucosa and gastroesophageal junction". Histology and Histopathology 17 (2): 445–54. PMID 11962749.
• Kanduc D (Jul 2002). "Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7". Journal of Virology 76 (14): 7040–8. doi:10.1128/JVI.76.14.7040-7048.2002. PMC 136328. PMID 12072504.
• Smith FJ, Porter RM, Corden LD, Lunny DP, Lane EB, McLean WH (Oct 2002). "Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse". Biochemical and Biophysical Research Communications 297 (4): 818–27. doi:10.1016/S0006-291X(02)02288-X. PMID 12359226.
• Blagoev B, Kratchmarova I, Ong SE, Nielsen M, Foster LJ, Mann M (Mar 2003). "A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling". Nature Biotechnology 21 (3): 315–8. doi:10.1038/nbt790. PMID 12577067.