CLIC4

Chloride intracellular channel 4
PDB rendering based on 2ahe.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2AHE​, 2D2Z​, 3OQS​
Identifiers
Symbols	CLIC4 ; CLIC4L; H1; MTCLIC; huH1; p64H1
External IDs	OMIM: 606536 MGI: 1352754 HomoloGene: 8490 GeneCards: CLIC4 Gene
Gene ontology Molecular function • glutathione transferase activity • voltage-gated ion channel activity • chloride channel activity • protein binding Cellular component • intracellular • cytoplasm • mitochondrion • centrosome • cytosol • plasma membrane • microvillus • cell-cell junction • cell surface • actin cytoskeleton • microtubule cytoskeleton • nuclear matrix • midbody • cytoplasmic vesicle membrane • chloride channel complex • apical part of cell • perinuclear region of cytoplasm • extracellular exosome Biological process • angiogenesis • endothelial cell morphogenesis • glutathione metabolic process • chloride transport • apoptotic process • vacuolar acidification • fertilization • cell differentiation • keratinocyte differentiation • negative regulation of cell migration • regulation of ion transmembrane transport • establishment or maintenance of apical/basal cell polarity • multicellular organism growth • branching morphogenesis of an epithelial tube • regulation of cytoskeleton organization • retina vasculature morphogenesis in camera-type eye • cellular response to calcium ion • chloride transmembrane transport Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	25932	29876
Ensembl	ENSG00000169504	ENSMUSG00000037242
UniProt	Q9Y696	Q9QYB1
RefSeq (mRNA)	NM_013943	NM_013885
RefSeq (protein)	NP_039234	NP_038913
Location (UCSC)	Chr 1: 24.75 – 24.84 Mb	Chr 4: 135.21 – 135.27 Mb
PubMed search

Chloride intracellular channel 4, also known as CLIC4, is a eukaryotic gene.^[1]

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).^[1]

Binding partners

CLIC4 binds to dynamin I, α-tubulin, β-actin, creatine kinase and two 14-3-3 isoforms.^[2]

See also

• Chloride channel

References

1. "Entrez Gene: CLIC4 chloride intracellular channel 4".
2. Suginta W, Karoulias N, Aitken A, Ashley RH. (October 2001). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.

Further reading

• Berryman M, Bretscher A (2000). "Identification of a Novel Member of the Chloride Intracellular Channel Gene Family (CLIC5) That Associates with the Actin Cytoskeleton of Placental Microvilli". Mol. Biol. Cell 11 (5): 1509–21. doi:10.1091/mbc.11.5.1509. PMC 14863. PMID 10793131.
• Berryman MA, Goldenring JR (2004). "CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells". Cell Motil. Cytoskeleton 56 (3): 159–72. doi:10.1002/cm.10141. PMID 14569596.
• Bohman S, Matsumoto T, Suh K; et al. (2006). "Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis". J. Biol. Chem. 280 (51): 42397–404. doi:10.1074/jbc.+M506724200. PMID 16239224.
• Chuang JZ, Milner TA, Zhu M, Sung CH (1999). "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons". J. Neurosci. 19 (8): 2919–28. PMID 10191309.
• Duncan RR, Westwood PK, Boyd A, Ashley RH (1997). "Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum". J. Biol. Chem. 272 (38): 23880–6. doi:10.1074/jbc.272.38.23880. PMID 9295337.
• Edwards JC (1999). "A novel p64-related Cl⁻ channel: subcellular distribution and nephron segment-specific expression". Am. J. Physiol. 276 (3 Pt 2): F398–408. PMID 10070163.
• Qian Z, Okuhara D, Abe MK, Rosner MR (1999). "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel". J. Biol. Chem. 274 (3): 1621–7. doi:10.1074/jbc.274.3.1621. PMID 9880541.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW (2002). "Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-β1-Mediated Conversion of Fibroblasts to Myofibroblasts". Am. J. Pathol. 161 (2): 471–80. doi:10.1016/S0002-9440(10)64203-4. PMC 1850723. PMID 12163372.
• Rual JF, Venkatesan K, Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Shiio Y, Suh KS, Lee H; et al. (2006). "Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4". J. Biol. Chem. 281 (5): 2750–6. doi:10.1074/jbc.+M509349200. PMID 16316993.
• Simpson JC, Wellenreuther R, Poustka A; et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Singh H, Ashley RH (2007). "CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels". Mol. Memb. Biol. 24 (1): 41–52. doi:10.1080/09687860600927907. PMID 17453412.
• Singh H, Cousin MA, Ashley RH (2007). "Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin". FEBS J. 274 (24): 6306–6316. doi:10.1111/j.1742-4658.2007.06145.x. PMID 18028448.
• Singh H (2010). "Two decades with dimorphic Chloride Intracellular Channels (CLICs)". FEBS Letters 584 (10): 2112–21. doi:10.1016/j.febslet.2010.03.013. PMID 20226783.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Suginta W, Karoulias N, Aitken A, Ashley RH (2003). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt 1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.
• Valenzuela SM, Martin DK, Por SB; et al. (1997). "Molecular cloning and expression of a chloride ion channel of cell nuclei". J. Biol. Chem. 272 (19): 12575–82. doi:10.1074/jbc.272.19.12575. PMID 9139710.
• Wick M, Bürger C, Brüsselbach S; et al. (1994). "Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression". J. Cell. Sci. 107 (1): 227–39. PMID 8175911.
• Wiemann S, Arlt D, Huber W; et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Wiemann S, Weil B, Wellenreuther R; et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.

External links

• CLIC4 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.