CDCP1

CUB domain containing protein 1
Identifiers
Symbols	CDCP1 ; CD318; SIMA135; TRASK
External IDs	OMIM: 611735 MGI: 2442010 HomoloGene: 11276 GeneCards: CDCP1 Gene
Gene ontology Molecular function • protein binding Cellular component • extracellular region • plasma membrane • integral component of membrane Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	64866	109332
Ensembl	ENSG00000163814	ENSMUSG00000035498
UniProt	Q9H5V8	Q5U462
RefSeq (mRNA)	NM_022842	NM_133974
RefSeq (protein)	NP_073753	NP_598735
Location (UCSC)	Chr 3: 45.08 – 45.15 Mb	Chr 9: 123.17 – 123.22 Mb
PubMed search

CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene.^[1][2] CDCP1 has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). Alternatively spliced transcript variants encoding distinct isoforms have been reported.^[2]

Function

CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion.^[3] The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

Clinical significance

The phosphorylation of CDCP1 is seen in many cancers, including some pre-invasive cancers as well as in invasive tumors and in tumor metastases. The functional implications of CDCP1 phosphorylation in tumors is currently under investigation.

References

1. Scherl-Mostageer M, Sommergruber W, Abseher R, Hauptmann R, Ambros P, Schweifer N (Jul 2001). "Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer". Oncogene 20 (32): 4402–8. doi:10.1038/sj.onc.1204566. PMID 11466621.
2. "Entrez Gene: CDCP1 CUB domain containing protein 1".
3. Spassov DS, Ahuja D, Wong CH, Moasser MM (2011). "The structural features of trask that mediate its anti-adhesive functions". PLoS ONE 6 (4): e19154. doi:10.1371/journal.pone.0019154. PMC 3084758. PMID 21559459.

Further reading

• Xia Y, Gil SG, Carter WG (1996). "Anchorage mediated by integrin alpha6beta4 to laminin 5 (epiligrin) regulates tyrosine phosphorylation of a membrane-associated 80-kD protein.". J. Cell Biol. 132 (4): 727–40. doi:10.1083/jcb.132.4.727. PMC 2199869. PMID 8647901.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Hooper JD, Zijlstra A, Aimes RT; et al. (2003). "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen.". Oncogene 22 (12): 1783–94. doi:10.1038/sj.onc.1206220. PMID 12660814.
• Conze T, Lammers R, Kuci S; et al. (2003). "CDCP1 is a novel marker for hematopoietic stem cells.". Ann. N. Y. Acad. Sci. 996 (1): 222–6. doi:10.1111/j.1749-6632.2003.tb03249.x. PMID 12799299.
• Clark HF, Gurney AL, Abaya E; et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Brown TA, Yang TM, Zaitsevskaia T; et al. (2004). "Adhesion or plasmin regulates tyrosine phosphorylation of a novel membrane glycoprotein p80/gp140/CUB domain-containing protein 1 in epithelia.". J. Biol. Chem. 279 (15): 14772–83. doi:10.1074/jbc.M309678200. PMID 14739293.
• Bühring HJ, Kuçi S, Conze T; et al. (2005). "CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin.". Stem Cells 22 (3): 334–43. doi:10.1634/stemcells.22-3-334. PMID 15153610.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Benes CH, Wu N, Elia AE; et al. (2005). "The C2 domain of PKCdelta is a phosphotyrosine binding domain.". Cell 121 (2): 271–80. doi:10.1016/j.cell.2005.02.019. PMID 15851033.
• Bhatt AS, Erdjument-Bromage H, Tempst P; et al. (2005). "Adhesion signaling by a novel mitotic substrate of src kinases.". Oncogene 24 (34): 5333–43. doi:10.1038/sj.onc.1208582. PMC 3023961. PMID 16007225.
• André M, Le Caer JP, Greco C; et al. (2006). "Proteomic analysis of the tetraspanin web using LC-ESI-MS/MS and MALDI-FTICR-MS.". Proteomics 6 (5): 1437–49. doi:10.1002/pmic.200500180. PMID 16404722.
• Kimura H, Morii E, Ikeda JI; et al. (2006). "Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells.". Leukemia 20 (9): 1551–6. doi:10.1038/sj.leu.2404312. PMID 16926850.
• Perry SE, Robinson P, Melcher A; et al. (2007). "Expression of the CUB domain containing protein 1 (CDCP1) gene in colorectal tumour cells.". FEBS Lett. 581 (6): 1137–42. doi:10.1016/j.febslet.2007.02.025. PMID 17335815.
• Uekita T, Jia L, Narisawa-Saito M; et al. (2007). "CUB domain-containing protein 1 is a novel regulator of anoikis resistance in lung adenocarcinoma.". Mol. Cell. Biol. 27 (21): 7649–60. doi:10.1128/MCB.01246-07. PMC 2169043. PMID 17785447.

External links

• CDCP1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.