E2F4

E2F transcription factor 4, p107/p130-binding
PDB rendering based on 1cf7.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1CF7​
Identifiers
Symbols	E2F4 ; E2F-4
External IDs	OMIM: 600659 MGI: 103012 HomoloGene: 1471 GeneCards: E2F4 Gene
Gene ontology Molecular function • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • DNA binding • transcription factor activity, sequence-specific DNA binding • protein binding • transcription factor binding • protein domain specific binding Cellular component • nucleoplasm • transcription factor complex Biological process • regulation of transcription involved in G1/S transition of mitotic cell cycle • mitotic cell cycle • epithelial cell development • transcription, DNA-templated • transcription initiation from RNA polymerase II promoter • cell volume homeostasis • transforming growth factor beta receptor signaling pathway • blood circulation • organ morphogenesis • gene expression • regulation of cell proliferation • motile cilium assembly • positive regulation of transcription from RNA polymerase II promoter • centriole assembly • multi-ciliated epithelial cell differentiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1874	104394
Ensembl	ENSG00000205250	ENSMUSG00000014859
UniProt	Q16254	Q8R0K9
RefSeq (mRNA)	NM_001950	NM_148952
RefSeq (protein)	NP_001941	NP_683754
Location (UCSC)	Chr 16: 67.19 – 67.2 Mb	Chr 8: 105.3 – 105.31 Mb
PubMed search

Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene.^[1][2]

Function

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.^[3]

Structure

The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids (Asp + Glu), and a tumor suppressor protein association domain which is embedded within the transactivation domain.

Interactions

E2F4 has been shown to interact with Smad3.^[4]

See also

• E2F

References

1. Ginsberg D, Vairo G, Chittenden T, Xiao ZX, Xu G, Wydner KL, DeCaprio JA, Lawrence JB, Livingston DM (Dec 1994). "E2F-4, a new member of the E2F transcription factor family, interacts with p107". Genes Dev 8 (22): 2665–79. doi:10.1101/gad.8.22.2665. PMID 7958924.
2. Sardet C, Vidal M, Cobrinik D, Geng Y, Onufryk C, Chen A, Weinberg RA (Apr 1995). "E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle". Proc Natl Acad Sci U S A 92 (6): 2403–7. doi:10.1073/pnas.92.6.2403. PMC 42492. PMID 7892279.
3. "Entrez Gene: E2F4 E2F transcription factor 4, p107/p130-binding".
4. Chen CR, Kang Y, Siegel PM, Massagué J (July 2002). "E2F4/5 and p107 as Smad cofactors linking the TGFbeta receptor to c-myc repression". Cell 110 (1): 19–32. doi:10.1016/S0092-8674(02)00801-2. PMID 12150994.

Further reading

• Bandyopadhyay D, Timchenko N, Suwa T; et al. (2001). "The human melanocyte: a model system to study the complexity of cellular aging and transformation in non-fibroblastic cells.". Exp. Gerontol. 36 (8): 1265–75. doi:10.1016/S0531-5565(01)00098-5. PMID 11602203.
• Beijersbergen RL, Kerkhoven RM, Zhu L; et al. (1994). "E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.". Genes Dev. 8 (22): 2680–90. doi:10.1101/gad.8.22.2680. PMID 7958925.
• Xiao ZX, Ginsberg D, Ewen M, Livingston DM (1996). "Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin-associated kinases.". Proc. Natl. Acad. Sci. U.S.A. 93 (10): 4633–7. doi:10.1073/pnas.93.10.4633. PMC 39330. PMID 8643455.
• Moberg K, Starz MA, Lees JA (1996). "E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry.". Mol. Cell. Biol. 16 (4): 1436–49. PMC 231128. PMID 8657117.
• Vidal M, Brachmann RK, Fattaey A; et al. (1996). "Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions.". Proc. Natl. Acad. Sci. U.S.A. 93 (19): 10315–20. doi:10.1073/pnas.93.19.10315. PMC 38381. PMID 8816797.
• Williams CD, Linch DC, Sørensen TS; et al. (1997). "The predominant E2F complex in human primary haemopoietic cells and in AML blasts contains E2F-4, DP-1 and p130.". Br. J. Haematol. 96 (4): 688–96. doi:10.1046/j.1365-2141.1997.d01-2086.x. PMID 9074408.
• Lindeman GJ, Gaubatz S, Livingston DM, Ginsberg D (1997). "The subcellular localization of E2F-4 is cell-cycle dependent.". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 5095–100. doi:10.1073/pnas.94.10.5095. PMC 24637. PMID 9144196.
• Wang H, Shao N, Ding QM; et al. (1997). "BRCA1 proteins are transported to the nucleus in the absence of serum and splice variants BRCA1a, BRCA1b are tyrosine phosphoproteins that associate with E2F, cyclins and cyclin dependent kinases.". Oncogene 15 (2): 143–57. doi:10.1038/sj.onc.1201252. PMID 9244350.
• Müller H, Moroni MC, Vigo E; et al. (1997). "Induction of S-phase entry by E2F transcription factors depends on their nuclear localization.". Mol. Cell. Biol. 17 (9): 5508–20. PMC 232399. PMID 9271426.
• Pierce AM, Schneider-Broussard R, Philhower JL, Johnson DG (1998). "Differential activities of E2F family members: unique functions in regulating transcription.". Mol. Carcinog. 22 (3): 190–8. doi:10.1002/(SICI)1098-2744(199807)22:3<190::AID-MC7>3.0.CO;2-P. PMID 9688145.
• Ferreira R, Magnaghi-Jaulin L, Robin P; et al. (1998). "The three members of the pocket proteins family share the ability to repress E2F activity through recruitment of a histone deacetylase.". Proc. Natl. Acad. Sci. U.S.A. 95 (18): 10493–8. doi:10.1073/pnas.95.18.10493. PMC 27922. PMID 9724731.
• Timchenko NA, Wilde M, Darlington GJ (1999). "C/EBPalpha regulates formation of S-phase-specific E2F-p107 complexes in livers of newborn mice.". Mol. Cell. Biol. 19 (4): 2936–45. PMC 84088. PMID 10082561.
• Zheng N, Fraenkel E, Pabo CO, Pavletich NP (1999). "Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP.". Genes Dev. 13 (6): 666–74. doi:10.1101/gad.13.6.666. PMC 316551. PMID 10090723.
• Furukawa Y, Iwase S, Kikuchi J; et al. (1999). "Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes.". Oncogene 18 (11): 2003–14. doi:10.1038/sj.onc.1202500. PMID 10208422.
• Lam EW, Glassford J, van der Sman J; et al. (1999). "Modulation of E2F activity in primary mouse B cells following stimulation via surface IgM and CD40 receptors.". Eur. J. Immunol. 29 (10): 3380–9. doi:10.1002/(SICI)1521-4141(199910)29:10<3380::AID-IMMU3380>3.0.CO;2-C. PMID 10540350.
• Zhong X, Hemmi H, Koike J; et al. (2000). "Various AGC repeat numbers in the coding region of the human transcription factor gene E2F-4.". Hum. Mutat. 15 (3): 296–7. doi:10.1002/(SICI)1098-1004(200003)15:3<296::AID-HUMU18>3.0.CO;2-X. PMID 10679953.
• Takahashi Y, Rayman JB, Dynlacht BD (2000). "Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression.". Genes Dev. 14 (7): 804–16. doi:10.1101/gad.14.7.804. PMC 316494. PMID 10766737.
• Schwemmle S, Pfeifer GP (2000). "Genomic structure and mutation screening of the E2F4 gene in human tumors.". Int. J. Cancer 86 (5): 672–7. doi:10.1002/(SICI)1097-0215(20000601)86:5<672::AID-IJC11>3.0.CO;2-X. PMID 10797289.

External links

• E2F4 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)
• FactorBook E2F4

This article incorporates text from the United States National Library of Medicine, which is in the public domain.