Y box binding protein 1

Y box binding protein 1
PDB rendering based on 1h95.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1H95​
Identifiers
Symbols	YBX1 ; BP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1
External IDs	OMIM: 154030 HomoloGene: 88707 GeneCards: YBX1 Gene
Gene ontology Molecular function • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • DNA binding • chromatin binding • double-stranded DNA binding • single-stranded DNA binding • transcription factor activity, sequence-specific DNA binding • RNA binding • protein binding • poly(A) RNA binding • GTPase binding Cellular component • nucleus • nucleoplasm • U12-type spliceosomal complex • cytoplasm • cytoplasmic stress granule • ribonucleoprotein complex • nuclear membrane • intracellular membrane-bounded organelle • extracellular exosome • CRD-mediated mRNA stability complex • histone pre-mRNA 3'end processing complex Biological process • negative regulation of transcription from RNA polymerase II promoter • mRNA splicing, via spliceosome • in utero embryonic development • regulation of transcription, DNA-templated • transcription from RNA polymerase II promoter • RNA splicing • gene expression • positive regulation of transcription from RNA polymerase II promoter • negative regulation of striated muscle cell differentiation • positive regulation of cell division • CRD-mediated mRNA stabilization Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4904	22608
Ensembl	ENSG00000065978	ENSMUSG00000028639
UniProt	P67809	P62960
RefSeq (mRNA)	NM_004559	NM_011732
RefSeq (protein)	NP_004550	NP_035862
Location (UCSC)	Chr 1: 42.68 – 42.7 Mb	Chr 4: 119.28 – 119.29 Mb
PubMed search

Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded by the YBX1 gene.^[1]

Clinical significance

YBX1 is a potential drug target in cancer therapy.^[2] YB-1 helps the replication of adenovirus type 5, a commonly used vector in gene therapy.^[2] Thus, YB-1 can cause an "oncolytic" effect in YB-1 positive cancer cells treated with adenoviruses

Interactions

Y box binding protein 1 has been shown to interact with:

• ANKRD2,^[3]
• CTCF,^[4]
• P53,^[3][5]
• PCNA,^[6]
• RBBP6,^[7] and
• SFRS9.^[8]

References

1. "Entrez Gene: YBX1 Y box binding protein 1".
2. Lage H, Surowiak P, Holm PS (November 2008). "[YB-1 as a potential target in cancer therapy]". Pathologe (in German). 29 Suppl 2: 187–90. doi:10.1007/s00292-008-1030-2. PMID 18773210.
3. Kojic S, Medeot E, Guccione E, Krmac H, Zara I, Martinelli V, Valle G, Faulkner G (2004). "The Ankrd2 protein, a link between the sarcomere and the nucleus in skeletal muscle". J. Mol. Biol. 339 (2): 313–25. doi:10.1016/j.jmb.2004.03.071. PMID 15136035.
4. Chernukhin IV, Shamsuddin S, Robinson AF, Carne AF, Paul A, El-Kady AI, Lobanenkov VV, Klenova EM (2000). "Physical and functional interaction between two pluripotent proteins, the Y-box DNA/RNA-binding factor, YB-1, and the multivalent zinc finger factor, CTCF". J. Biol. Chem. 275 (38): 29915–21. doi:10.1074/jbc.M001538200. PMID 10906122.
5. Okamoto T, Izumi H, Imamura T, Takano H, Ise T, Uchiumi T, Kuwano M, Kohno K (2000). "Direct interaction of p53 with the Y-box binding protein, YB-1: a mechanism for regulation of human gene expression". Oncogene 19 (54): 6194–202. doi:10.1038/sj.onc.1204029. PMID 11175333.
6. Ise T, Nagatani G, Imamura T, Kato K, Takano H, Nomoto M, Izumi H, Ohmori H, Okamoto T, Ohga T, Uchiumi T, Kuwano M, Kohno K (1999). "Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen". Cancer Res. 59 (2): 342–6. PMID 9927044.
7. Chibi M, Meyer M, Skepu A, G Rees DJ, Moolman-Smook JC, Pugh DJ (2008). "RBBP6 interacts with multifunctional protein YB-1 through its RING finger domain, leading to ubiquitination and proteosomal degradation of YB-1". J. Mol. Biol. 384 (4): 908–16. doi:10.1016/j.jmb.2008.09.060. PMID 18851979.
8. Raffetseder U, Frye B, Rauen T, Jürchott K, Royer HD, Jansen PL, Mertens PR (2003). "Splicing factor SRp30c interaction with Y-box protein-1 confers nuclear YB-1 shuttling and alternative splice site selection". J. Biol. Chem. 278 (20): 18241–8. doi:10.1074/jbc.M212518200. PMID 12604611.

Further reading

• Kolluri R, Torrey TA, Kinniburgh AJ (1992). "A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif.". Nucleic Acids Res. 20 (1): 111–6. doi:10.1093/nar/20.1.111. PMC 310333. PMID 1738588.
• Kolluri R, Kinniburgh AJ (1991). "Full length cDNA sequence encoding a nuclease-sensitive element DNA binding protein.". Nucleic Acids Res. 19 (17): 4771. doi:10.1093/nar/19.17.4771. PMC 328725. PMID 1891370.
• Sakura H, Maekawa T, Imamoto F, et al. (1989). "Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology.". Gene 73 (2): 499–507. doi:10.1016/0378-1119(88)90514-8. PMID 2977358.
• Didier DK, Schiffenbauer J, Woulfe SL, et al. (1988). "Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.". Proc. Natl. Acad. Sci. U.S.A. 85 (19): 7322–6. doi:10.1073/pnas.85.19.7322. PMC 282178. PMID 3174636.
• Kudo S, Mattei MG, Fukuda M (1995). "Characterization of the gene for dbpA, a family member of the nucleic-acid-binding proteins containing a cold-shock domain.". Eur. J. Biochem. 231 (1): 72–82. doi:10.1111/j.1432-1033.1995.tb20672.x. PMID 7628487.
• Lloberas J, Maki RA, Celada A (1995). "Repression of major histocompatibility complex I-A beta gene expression by dbpA and dbpB (mYB-1) proteins.". Mol. Cell. Biol. 15 (9): 5092–9. PMC 230756. PMID 7651426.
• Chen NN, Chang CF, Gallia GL, et al. (1995). "Cooperative action of cellular proteins YB-1 and Pur alpha with the tumor antigen of the human JC polyomavirus determines their interaction with the viral lytic control element.". Proc. Natl. Acad. Sci. U.S.A. 92 (4): 1087–91. doi:10.1073/pnas.92.4.1087. PMC 42642. PMID 7862639.
• Horwitz EM, Maloney KA, Ley TJ (1994). "A human protein containing a "cold shock" domain binds specifically to H-DNA upstream from the human gamma-globin genes.". J. Biol. Chem. 269 (19): 14130–9. PMID 8188694.
• Kashanchi F, Duvall JF, Dittmer J, et al. (1994). "Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type I basal gene expression.". J. Virol. 68 (1): 561–5. PMC 236322. PMID 8254772.
• Makino Y, Ohga T, Toh S, et al. (1996). "Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter.". Nucleic Acids Res. 24 (10): 1873–8. doi:10.1093/nar/24.10.1873. PMC 145870. PMID 8657568.
• Funke B, Zuleger B, Benavente R, et al. (1996). "The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins.". Nucleic Acids Res. 24 (19): 3821–8. doi:10.1093/nar/24.19.3821. PMC 146158. PMID 8871564.
• Koike K, Uchiumi T, Ohga T, et al. (1998). "Nuclear translocation of the Y-box binding protein by ultraviolet irradiation.". FEBS Lett. 417 (3): 390–4. doi:10.1016/S0014-5793(97)01296-9. PMID 9409758.
• Toh S, Nakamura T, Ohga T, et al. (1998). "Genomic organization of the human Y-box protein (YB-1) gene.". Gene 206 (1): 93–7. doi:10.1016/S0378-1119(97)00570-2. PMID 9461420.
• Sawaya BE, Khalili K, Amini S (1998). "Transcription of the human immunodeficiency virus type 1 (HIV-1) promoter in central nervous system cells: effect of YB-1 on expression of the HIV-1 long terminal repeat.". J. Gen. Virol. 79. ( Pt 2): 239–46. PMID 9472608.
• Mertens PR, Alfonso-Jaume MA, Steinmann K, Lovett DH (1999). "A synergistic interaction of transcription factors AP2 and YB-1 regulates gelatinase A enhancer-dependent transcription.". J. Biol. Chem. 273 (49): 32957–65. doi:10.1074/jbc.273.49.32957. PMID 9830047.
• Ise T, Nagatani G, Imamura T, et al. (1999). "Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen.". Cancer Res. 59 (2): 342–6. PMID 9927044.
• Safak M, Gallia GL, Khalili K (1999). "Reciprocal interaction between two cellular proteins, Puralpha and YB-1, modulates transcriptional activity of JCVCY in glial cells.". Mol. Cell. Biol. 19 (4): 2712–23. PMC 84064. PMID 10082537.
• Kelm RJ, Cogan JG, Elder PK, et al. (1999). "Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter.". J. Biol. Chem. 274 (20): 14238–45. doi:10.1074/jbc.274.20.14238. PMID 10318844.
• Safak M, Gallia GL, Ansari SA, Khalili K (1999). "Physical and functional interaction between the Y-box binding protein YB-1 and human polyomavirus JC virus large T antigen.". J. Virol. 73 (12): 10146–57. PMC 113067. PMID 10559330.
• Ansari SA, Safak M, Gallia GL, et al. (1999). "Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity.". J. Gen. Virol. 80. ( Pt 10): 2629–38. PMID 10573156.
• Basaki Y, Hosoi F, Oda Y, et al. (2007). "Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells.". Oncogene 26 (19): 2736–46. doi:10.1038/sj.onc.1210084. PMID 17072343.

External links

• YBX1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.