PBX1

Pre-B-cell leukemia homeobox 1
Cartoon diagram of a HOXB1–PBX1 heterodimer (violet) binding a DNA fragment (blue). Rendering based on PDB 1b72.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1B72​, 1PUF​
Identifiers
Symbols	PBX1 ; DKFZp686B09108; MGC126627
External IDs	OMIM: 176310 MGI: 97495 HomoloGene: 20574 ChEMBL: 4587 GeneCards: PBX1 Gene
Gene ontology Molecular function • RNA polymerase II core promoter proximal region sequence-specific DNA binding • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding • DNA binding • transcription factor activity, sequence-specific DNA binding • protein binding • transcription factor binding • protein heterodimerization activity Cellular component • nucleus • nucleoplasm • transcription factor complex • cytoplasm Biological process • branching involved in ureteric bud morphogenesis • transcription from RNA polymerase II promoter • steroid biosynthetic process • sex differentiation • positive regulation of cell proliferation • anterior/posterior pattern specification • proximal/distal pattern formation • positive regulation of G2/M transition of mitotic cell cycle • regulation of ossification • adrenal gland development • embryonic limb morphogenesis • somatic stem cell maintenance • embryonic hemopoiesis • negative regulation of sequence-specific DNA binding transcription factor activity • negative regulation of neuron differentiation • positive regulation of transcription from RNA polymerase II promoter • spleen development • thymus development • embryonic skeletal system development Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5087	18514
Ensembl	ENSG00000185630	ENSMUSG00000052534
UniProt	P40424	P41778
RefSeq (mRNA)	NM_001204961	NM_001291508
RefSeq (protein)	NP_001191890	NP_001278437
Location (UCSC)	Chr 1: 164.56 – 164.9 Mb	Chr 1: 168.12 – 168.43 Mb
PubMed search

Pre-B-cell leukemia transcription factor 1 is a protein that in humans is encoded by the PBX1 gene.^[1]

Interactions

PBX1 has been shown to interact with:

• HOXB1,^[2][3]
• HOXB7,^[4]
• MEIS1,^[5][6][7] and
• Prep1.^[8]

References

1. "Entrez Gene: PBX1 Pre-B-cell leukemia homeobox 1".
2. Berthelsen J, Zappavigna V, Ferretti E, Mavilio F, Blasi F (Mar 1998). "The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity". The EMBO Journal 17 (5): 1434–45. doi:10.1093/emboj/17.5.1434. PMC 1170491. PMID 9482740.
3. Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C (Feb 1999). "Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation". Cell 96 (4): 587–97. doi:10.1016/S0092-8674(00)80662-5. PMID 10052460.
4. Chang CP, Shen WF, Rozenfeld S, Lawrence HJ, Largman C, Cleary ML (Mar 1995). "Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins". Genes & Development 9 (6): 663–74. doi:10.1101/gad.9.6.663. PMID 7729685.
5. Shen WF, Rozenfeld S, Kwong A, Köm ves LG, Lawrence HJ, Largman C (Apr 1999). "HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells". Molecular and Cellular Biology 19 (4): 3051–61. PMC 84099. PMID 10082572.
6. Shanmugam K, Green NC, Rambaldi I, Saragovi HU, Featherstone MS (Nov 1999). "PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins". Molecular and Cellular Biology 19 (11): 7577–88. PMC 84774. PMID 10523646.
7. Jacobs Y, Schnabel CA, Cleary ML (Jul 1999). "Trimeric association of Hox and TALE homeodomain proteins mediates Hoxb2 hindbrain enhancer activity". Molecular and Cellular Biology 19 (7): 5134–42. PMC 84356. PMID 10373562.
8. Zubair M, Ishihara S, Oka S, Okumura K, Morohashi K (Jun 2006). "Two-step regulation of Ad4BP/SF-1 gene transcription during fetal adrenal development: initiation by a Hox-Pbx1-Prep1 complex and maintenance via autoregulation by Ad4BP/SF-1". Molecular and Cellular Biology 26 (11): 4111–21. doi:10.1128/MCB.00222-06. PMC 1489093. PMID 16705164.

Further reading

• Sanyal M, Tung JW, Karsunky H, Zeng H, Selleri L, Weissman IL, Herzenberg LA, Cleary ML (May 2007). "B-cell development fails in the absence of the Pbx1 proto-oncogene". Blood 109 (10): 4191–9. doi:10.1182/blood-2006-10-054213. PMC 1885499. PMID 17244677.
• Hui H, Perfetti R (Feb 2002). "Pancreas duodenum homeobox-1 regulates pancreas development during embryogenesis and islet cell function in adulthood". European Journal of Endocrinology / European Federation of Endocrine Societies 146 (2): 129–41. doi:10.1530/eje.0.1460129. PMID 11834421.
• Hunger SP, Galili N, Carroll AJ, Crist WM, Link MP, Cleary ML (Feb 1991). "The t(1;19)(q23;p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias". Blood 77 (4): 687–93. PMID 1671560.
• Monica K, Galili N, Nourse J, Saltman D, Cleary ML (Dec 1991). "PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1". Molecular and Cellular Biology 11 (12): 6149–57. doi:10.1128/mcb.11.12.6149. PMC 361792. PMID 1682799.
• Nourse J, Mellentin JD, Galili N, Wilkinson J, Stanbridge E, Smith SD, Cleary ML (Feb 1990). "Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor". Cell 60 (4): 535–45. doi:10.1016/0092-8674(90)90657-Z. PMID 1967982.
• Kamps MP, Murre C, Sun XH, Baltimore D (Feb 1990). "A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL". Cell 60 (4): 547–55. doi:10.1016/0092-8674(90)90658-2. PMID 1967983.
• Lu Q, Wright DD, Kamps MP (Jun 1994). "Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation". Molecular and Cellular Biology 14 (6): 3938–48. doi:10.1128/mcb.14.6.3938. PMC 358760. PMID 7910944.
• Van Dijk MA, Voorhoeve PM, Murre C (Jul 1993). "Pbx1 is converted into a transcriptional activator upon acquiring the N-terminal region of E2A in pre-B-cell acute lymphoblastoid leukemia". Proceedings of the National Academy of Sciences of the United States of America 90 (13): 6061–5. doi:10.1073/pnas.90.13.6061. PMC 46867. PMID 8327485.
• Lu Q, Kamps MP (Apr 1996). "Structural determinants within Pbx1 that mediate cooperative DNA binding with pentapeptide-containing Hox proteins: proposal for a model of a Pbx1-Hox-DNA complex". Molecular and Cellular Biology 16 (4): 1632–40. doi:10.1128/mcb.16.4.1632. PMC 231149. PMID 8657138.
• Berthelsen J, Zappavigna V, Mavilio F, Blasi F (Mar 1998). "Prep1, a novel functional partner of Pbx proteins". The EMBO Journal 17 (5): 1423–33. doi:10.1093/emboj/17.5.1423. PMC 1170490. PMID 9482739.
• Berthelsen J, Zappavigna V, Ferretti E, Mavilio F, Blasi F (Mar 1998). "The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity". The EMBO Journal 17 (5): 1434–45. doi:10.1093/emboj/17.5.1434. PMC 1170491. PMID 9482740.
• Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C (Feb 1999). "Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation". Cell 96 (4): 587–97. doi:10.1016/S0092-8674(00)80662-5. PMID 10052460.
• Shen WF, Rozenfeld S, Kwong A, Köm ves LG, Lawrence HJ, Largman C (Apr 1999). "HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells". Molecular and Cellular Biology 19 (4): 3051–61. PMC 84099. PMID 10082572.
• Jacobs Y, Schnabel CA, Cleary ML (Jul 1999). "Trimeric association of Hox and TALE homeodomain proteins mediates Hoxb2 hindbrain enhancer activity". Molecular and Cellular Biology 19 (7): 5134–42. PMC 84356. PMID 10373562.
• Knoepfler PS, Bergstrom DA, Uetsuki T, Dac-Korytko I, Sun YH, Wright WE, Tapscott SJ, Kamps MP (Sep 1999). "A conserved motif N-terminal to the DNA-binding domains of myogenic bHLH transcription factors mediates cooperative DNA binding with pbx-Meis1/Prep1". Nucleic Acids Research 27 (18): 3752–61. doi:10.1093/nar/27.18.3752. PMC 148632. PMID 10471746.
• McWhirter JR, Neuteboom ST, Wancewicz EV, Monia BP, Downing JR, Murre C (Sep 1999). "Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia". Proceedings of the National Academy of Sciences of the United States of America 96 (20): 11464–9. doi:10.1073/pnas.96.20.11464. PMC 18056. PMID 10500199.
• Shanmugam K, Green NC, Rambaldi I, Saragovi HU, Featherstone MS (Nov 1999). "PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins". Molecular and Cellular Biology 19 (11): 7577–88. PMC 84774. PMID 10523646.
• Mikkola I, Bruun JA, Holm T, Johansen T (Feb 2001). "Superactivation of Pax6-mediated transactivation from paired domain-binding sites by dna-independent recruitment of different homeodomain proteins". The Journal of Biological Chemistry 276 (6): 4109–18. doi:10.1074/jbc.M008882200. PMID 11069920.
• Thameem F, Wolford JK, Bogardus C, Prochazka M (Mar 2001). "Analysis of PBX1 as a candidate gene for type 2 diabetes mellitus in Pima Indians". Biochimica Et Biophysica Acta 1518 (1-2): 215–20. doi:10.1016/S0167-4781(01)00189-0. PMID 11267683.
• Liu Y, MacDonald RJ, Swift GH (May 2001). "DNA binding and transcriptional activation by a PDX1.PBX1b.MEIS2b trimer and cooperation with a pancreas-specific basic helix-loop-helix complex". The Journal of Biological Chemistry 276 (21): 17985–93. doi:10.1074/jbc.M100678200. PMID 11279116.
• Mal A, Sturniolo M, Schiltz RL, Ghosh MK, Harter ML (Apr 2001). "A role for histone deacetylase HDAC1 in modulating the transcriptional activity of MyoD: inhibition of the myogenic program". The EMBO Journal 20 (7): 1739–53. doi:10.1093/emboj/20.7.1739. PMC 145490. PMID 11285237.

External links

• PBX1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)