FHL3

Four and a half LIM domains 3
PDB rendering based on 1wyh.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1WYH​, 2CUQ​, 2EHE​
Identifiers
Symbols	FHL3 ; SLIM2
External IDs	OMIM: 602790 MGI: 1341092 HomoloGene: 37928 GeneCards: FHL3 Gene
Gene ontology Molecular function • actin binding • protein binding • zinc ion binding Cellular component • stress fiber • nucleus • focal adhesion • Z disc Biological process • muscle organ development • actin cytoskeleton organization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2275	14201
Ensembl	ENSG00000183386	ENSMUSG00000032643
UniProt	Q13643	Q9R059
RefSeq (mRNA)	NM_001243878	NM_010213
RefSeq (protein)	NP_001230807	NP_034343
Location (UCSC)	Chr 1: 38 – 38.01 Mb	Chr 4: 124.7 – 124.71 Mb
PubMed search

Four and a half LIM domains protein 3 is a protein that in humans is encoded by the FHL3 gene.^[1][2][3]

LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]^[3]

Interactions

FHL3 has been shown to interact with:

• CREB1,^[4]
• CTBP2,^[5]
• FHL2,^[4][6]
• ITGA7^[7] and
• KLF3.^[5]

References

1. Morgan MJ, Madgwick AJ (October 1996). "Slim defines a novel family of LIM-proteins expressed in skeletal muscle". Biochem Biophys Res Commun 225 (2): 632–8. doi:10.1006/bbrc.1996.1222. PMID 8753811.
2. Lee SM, Tsui SK, Chan KK, Kotaka M, Li HY, Chim SS, Waye MM, Fung KP, Lee CY (May 1999). "Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1". Somat Cell Mol Genet 24 (3): 197–202. doi:10.1023/B:SCAM.0000007122.03392.4b. PMID 10226657.
3. "Entrez Gene: FHL3 four and a half LIM domains 3".
4. Fimia GM, De Cesare D, Sassone-Corsi P (November 2000). "A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM". Mol. Cell. Biol. 20 (22): 8613–22. doi:10.1128/mcb.20.22.8613-8622.2000. PMC 102166. PMID 11046156.
5. Turner J, Nicholas H, Bishop D, Matthews JM, Crossley M (April 2003). "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". J. Biol. Chem. 278 (15): 12786–95. doi:10.1074/jbc.M300587200. PMID 12556451.
6. Li HY, Ng EK, Lee SM, Kotaka M, Tsui SK, Lee CY, Fung KP, Waye MM (2001). "Protein–protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)". J. Cell. Biochem. 80 (3): 293–303. doi:10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U. PMID 11135358.
7. Samson T, Smyth N, Janetzky S, Wendler O, Müller JM, Schüle R, von der Mark H, von der Mark K, Wixler V (July 2004). "The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor". J. Biol. Chem. 279 (27): 28641–52. doi:10.1074/jbc.M312894200. PMID 15117962.

Further reading

• Morgan MJ, Madgwick AJ (1999). "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle.". Biochem. Biophys. Res. Commun. 255 (2): 245–50. doi:10.1006/bbrc.1999.0179. PMID 10049693.
• Li HY, Ng EK, Lee SM, Kotaka M, Tsui SK, Lee CY, Fung KP, Waye MM (2001). "Protein–protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET).". J. Cell. Biochem. 80 (3): 293–303. doi:10.1002/1097-4644(20010301)80:3<293::AID-JCB10>3.0.CO;2-U. PMID 11135358.
• Turner J, Nicholas H, Bishop D, Matthews JM, Crossley M (2003). "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2.". J. Biol. Chem. 278 (15): 12786–95. doi:10.1074/jbc.M300587200. PMID 12556451.
• Mils V, Lee SM, Joly W, Hang EW, Baldin V, Waye MM, Ducommun B, Tsui SK (2003). "LIM-only protein FHL3 interacts with CDC25B2 phosphatase.". Exp. Cell Res. 285 (1): 99–106. doi:10.1016/S0014-4827(03)00018-1. PMID 12681290.
• Coghill ID, Brown S, Cottle DL, McGrath MJ, Robinson PA, Nandurkar HH, Dyson JM, Mitchell CA (2003). "FHL3 is an actin-binding protein that regulates alpha-actinin-mediated actin bundling: FHL3 localizes to actin stress fibers and enhances cell spreading and stress fiber disassembly.". J. Biol. Chem. 278 (26): 24139–52. doi:10.1074/jbc.M213259200. PMID 12704194.
• Purcell NH, Darwis D, Bueno OF, Müller JM, Schüle R, Molkentin JD (2004). "Extracellular signal-regulated kinase 2 interacts with and is negatively regulated by the LIM-only protein FHL2 in cardiomyocytes.". Mol. Cell. Biol. 24 (3): 1081–95. doi:10.1128/MCB.24.3.1081-1095.2004. PMC 321437. PMID 14729955.
• Samson T, Smyth N, Janetzky S, Wendler O, Müller JM, Schüle R, von der Mark H, von der Mark K, Wixler V (2004). "The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor.". J. Biol. Chem. 279 (27): 28641–52. doi:10.1074/jbc.M312894200. PMID 15117962.
• Takahashi K, Matsumoto C, Ra C (2005). "FHL3 negatively regulates human high-affinity IgE receptor beta-chain gene expression by acting as a transcriptional co-repressor of MZF-1.". Biochem. J. 386 (Pt 1): 191–200. doi:10.1042/BJ20040775. PMC 1134781. PMID 15453830.
• Philippar U, Schratt G, Dieterich C, Müller JM, Galgóczy P, Engel FB, Keating MT, Gertler F, Schüle R, Vingron M, Nordheim A (2005). "The SRF target gene Fhl2 antagonizes RhoA/MAL-dependent activation of SRF.". Mol. Cell 16 (6): 867–80. doi:10.1016/j.molcel.2004.11.039. PMID 15610731.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein–protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Cottle DL, McGrath MJ, Cowling BS, Coghill ID, Brown S, Mitchell CA (2007). "FHL3 binds MyoD and negatively regulates myotube formation.". J. Cell. Sci. 120 (Pt 8): 1423–35. doi:10.1242/jcs.004739. PMID 17389685.

External links

• FHL3 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.