IKZF1

IKAROS family zinc finger 1 (Ikaros)
Identifiers
Symbols	IKZF1 ; Hs.54452; IK1; IKAROS; LYF1; LyF-1; PPP1R92; PRO0758; ZNFN1A1
External IDs	OMIM: 603023 MGI: 1342540 HomoloGene: 55948 GeneCards: IKZF1 Gene
Gene ontology Molecular function • DNA binding • transcription factor activity, sequence-specific DNA binding • protein binding • sequence-specific DNA binding • metal ion binding • protein heterodimerization activity Cellular component • nucleus • pericentric heterochromatin • cytoplasm • protein complex Biological process • negative regulation of transcription from RNA polymerase II promoter • natural killer cell differentiation • transcription, DNA-templated • cell cycle • mesoderm development • chromatin modification • lymphocyte differentiation • B cell differentiation • T cell differentiation • erythrocyte differentiation • forebrain development • positive regulation of multicellular organism growth • positive regulation of B cell differentiation • positive regulation of neutrophil differentiation • negative regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • lymph node development • thymus development • Peyer's patch development • positive regulation of NK T cell differentiation • retina development in camera-type eye Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10320	22778
Ensembl	ENSG00000185811	ENSMUSG00000018654
UniProt	Q13422	Q03267
RefSeq (mRNA)	NM_001220765	NM_001025597
RefSeq (protein)	NP_001207694	NP_001020768
Location (UCSC)	Chr 7: 50.3 – 50.41 Mb	Chr 11: 11.69 – 11.77 Mb
PubMed search

DNA-binding protein Ikaros also known as Ikaros family zinc finger protein 1 is a protein that in humans is encoded by the IKZF1 gene.^[1][2][3] Ikaros displays crucial functions in the hematopoietic system and its loss of function has been linked to the development of lymphoid leukemia.^[4] In particular, Ikaros has been found in recent years to be a major tumor suppressor involved in human B-cell acute lymphoblastic leukemia.^[4] IKZF1 is upregulated in granulocytes, B cells, CD4 and CD8 T cells, and NK cells, and downregulated in erythroblasts, megakaryocytes and monocytes.^[5] In Ikaros knockout mice, T cells but not B cells are generated late in mouse development due to late compensatory expression of the related gene Aiolos (IKZF3).^[6] Ikaros point mutant mice are embryonic lethal due to anemia; they have severe defects in terminal erythrocyte and granulocyte differentiation, and excessive macrophage formation.^[7] SNPs located near the 3' region of IKZF1 in humans have been linked to susceptibility to childhood acute lymphoblastic leukemia (ALL) ^[8] as well as type 1 diabetes.^[9] The two effects appear to be in opposite directions, with the allele marking susceptibility to ALL protecting from T1D and vice versa.^[9]

Interactions

IKZF1 has been shown to interact with:

• CTBP1,^[10]
• HDAC1,^[11][12]
• HDAC7A,^[12]
• Histone deacetylase 5,^[12]
• IKZF2,^[13]
• IKZF3,^[14][11][15]
• IKZF4,^[16][17]
• SIN3A,^[11][12][18] and
• SIN3B.^[11][12]

References

1. Georgopoulos K, Moore DD, Derfler B (Oct 1992). "Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment". Science 258 (5083): 808–12. doi:10.1126/science.1439790. PMID 1439790.
2. Hahm K, Ernst P, Lo K, Kim GS, Turck C, Smale ST (Nov 1994). "The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene". Molecular and Cellular Biology 14 (11): 7111–23. doi:10.1128/mcb.14.11.7111. PMC 359245. PMID 7935426.
3. "Entrez Gene: IKZF1 IKAROS family zinc finger 1 (Ikaros)".
4. Kastner P, Chan S (Jun 2011). "Role of Ikaros in T-cell acute lymphoblastic leukemia". World Journal of Biological Chemistry 2 (6): 108–14. doi:10.4331/wjbc.v2.i6.108. PMC 3135856. PMID 21765975.
5. Watkins NA, Gusnanto A, de Bono B, De S, Miranda-Saavedra D, Hardie DL, Angenent WG, Attwood AP, Ellis PD, Erber W, Foad NS, Garner SF, Isacke CM, Jolley J, Koch K, Macaulay IC, Morley SL, Rendon A, Rice KM, Taylor N, Thijssen-Timmer DC, Tijssen MR, van der Schoot CE, Wernisch L, Winzer T, Dudbridge F, Buckley CD, Langford CF, Teichmann S, Göttgens B, Ouwehand WH (May 2009). "A HaemAtlas: characterizing gene expression in differentiated human blood cells". Blood 113 (19): e1–9. doi:10.1182/blood-2008-06-162958. PMC 2680378. PMID 19228925.
6. Georgopoulos K, Winandy S, Avitahl N (1997). "The role of the Ikaros gene in lymphocyte development and homeostasis". Annual Review of Immunology 15: 155–76. doi:10.1146/annurev.immunol.15.1.155. PMID 9143685.
7. Papathanasiou P, Perkins AC, Cobb BS, Ferrini R, Sridharan R, Hoyne GF, Nelms KA, Smale ST, Goodnow CC (July 2003). "Widespread failure of hematolymphoid differentiation caused by a recessive niche-filling allele of the Ikaros transcription factor". Immunity 19 (1): 131–44. doi:10.1016/s1074-7613(03)00168-7.
8. Papaemmanuil E, Hosking FJ, Vijayakrishnan J, Price A, Olver B, Sheridan E, Kinsey SE, Lightfoot T, Roman E, Irving JA, Allan JM, Tomlinson IP, Taylor M, Greaves M, Houlston RS (Sep 2009). "Loci on 7p12.2, 10q21.2 and 14q11.2 are associated with risk of childhood acute lymphoblastic leukemia". Nature Genetics 41 (9): 1006–10. doi:10.1038/ng.430. PMID 19684604.
9. Swafford AD, Howson JM, Davison LJ, Wallace C, Smyth DJ, Schuilenburg H, Maisuria-Armer M, Mistry T, Lenardo MJ, Todd JA (Mar 2011). "An allele of IKZF1 (Ikaros) conferring susceptibility to childhood acute lymphoblastic leukemia protects against type 1 diabetes". Diabetes 60 (3): 1041–4. doi:10.2337/db10-0446. PMC 3046822. PMID 21270240.
10. Koipally J, Georgopoulos K (Jun 2000). "Ikaros interactions with CtBP reveal a repression mechanism that is independent of histone deacetylase activity". The Journal of Biological Chemistry 275 (26): 19594–602. doi:10.1074/jbc.M000254200. PMID 10766745.
11. Koipally J, Renold A, Kim J, Georgopoulos K (Jun 1999). "Repression by Ikaros and Aiolos is mediated through histone deacetylase complexes". The EMBO Journal 18 (11): 3090–100. doi:10.1093/emboj/18.11.3090. PMC 1171390. PMID 10357820.
12. Koipally J, Georgopoulos K (Aug 2002). "A molecular dissection of the repression circuitry of Ikaros". The Journal of Biological Chemistry 277 (31): 27697–705. doi:10.1074/jbc.M201694200. PMID 12015313.
13. Kelley CM, Ikeda T, Koipally J, Avitahl N, Wu L, Georgopoulos K, Morgan BA (Apr 1998). "Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors". Current Biology 8 (9): 508–15. doi:10.1016/s0960-9822(98)70202-7. PMID 9560339.
14. Morgan B, Sun L, Avitahl N, Andrikopoulos K, Ikeda T, Gonzales E, Wu P, Neben S, Georgopoulos K (Apr 1997). "Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation". The EMBO Journal 16 (8): 2004–13. doi:10.1093/emboj/16.8.2004. PMC 1169803. PMID 9155026.
15. Kim J, Sif S, Jones B, Jackson A, Koipally J, Heller E, Winandy S, Viel A, Sawyer A, Ikeda T, Kingston R, Georgopoulos K (Mar 1999). "Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes". Immunity 10 (3): 345–55. doi:10.1016/s1074-7613(00)80034-5. PMID 10204490.
16. Honma Y, Kiyosawa H, Mori T, Oguri A, Nikaido T, Kanazawa K, Tojo M, Takeda J, Tanno Y, Yokoya S, Kawabata I, Ikeda H, Wanaka A (Mar 1999). "Eos: a novel member of the Ikaros gene family expressed predominantly in the developing nervous system". FEBS Letters 447 (1): 76–80. doi:10.1016/s0014-5793(99)00265-3. PMID 10218586.
17. Perdomo J, Holmes M, Chong B, Crossley M (Dec 2000). "Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities". The Journal of Biological Chemistry 275 (49): 38347–54. doi:10.1074/jbc.M005457200. PMID 10978333.
18. Koipally J, Georgopoulos K (Jun 2002). "Ikaros-CtIP interactions do not require C-terminal binding protein and participate in a deacetylase-independent mode of repression". The Journal of Biological Chemistry 277 (26): 23143–9. doi:10.1074/jbc.M202079200. PMID 11959865.

Further reading

• Tonnelle C, Calmels B, Maroc C, Gabert J, Chabannon C (Jan 2002). "Ikaros gene expression and leukemia". Leukemia & Lymphoma 43 (1): 29–35. doi:10.1080/10428190210186. PMID 11908734.
• Westman BJ, Mackay JP, Gell D (Oct 2002). "Ikaros: a key regulator of haematopoiesis". The International Journal of Biochemistry & Cell Biology 34 (10): 1304–7. doi:10.1016/S1357-2725(02)00070-5. PMID 12127581.
• Molnár A, Wu P, Largespada DA, Vortkamp A, Scherer S, Copeland NG, Jenkins NA, Bruns G, Georgopoulos K (Jan 1996). "The Ikaros gene encodes a family of lymphocyte-restricted zinc finger DNA binding proteins, highly conserved in human and mouse". Journal of Immunology 156 (2): 585–92. PMID 8543809.
• Nietfeld W, Meyerhans A (Jan 1996). "Cloning and sequencing of hIk-1, a cDNA encoding a human homologue of mouse Ikaros/LyF-1". Immunology Letters 49 (1-2): 139–41. doi:10.1016/0165-2478(95)02479-4. PMID 8964602.
• Morgan B, Sun L, Avitahl N, Andrikopoulos K, Ikeda T, Gonzales E, Wu P, Neben S, Georgopoulos K (Apr 1997). "Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation". The EMBO Journal 16 (8): 2004–13. doi:10.1093/emboj/16.8.2004. PMC 1169803. PMID 9155026.
• Kelley CM, Ikeda T, Koipally J, Avitahl N, Wu L, Georgopoulos K, Morgan BA (Apr 1998). "Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors". Current Biology 8 (9): 508–15. doi:10.1016/S0960-9822(98)70202-7. PMID 9560339.
• Sun L, Heerema N, Crotty L, Wu X, Navara C, Vassilev A, Sensel M, Reaman GH, Uckun FM (Jan 1999). "Expression of dominant-negative and mutant isoforms of the antileukemic transcription factor Ikaros in infant acute lymphoblastic leukemia". Proceedings of the National Academy of Sciences of the United States of America 96 (2): 680–5. doi:10.1073/pnas.96.2.680. PMC 15196. PMID 9892693.
• Kim J, Sif S, Jones B, Jackson A, Koipally J, Heller E, Winandy S, Viel A, Sawyer A, Ikeda T, Kingston R, Georgopoulos K (Mar 1999). "Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes". Immunity 10 (3): 345–55. doi:10.1016/S1074-7613(00)80034-5. PMID 10204490.
• Honma Y, Kiyosawa H, Mori T, Oguri A, Nikaido T, Kanazawa K, Tojo M, Takeda J, Tanno Y, Yokoya S, Kawabata I, Ikeda H, Wanaka A (Mar 1999). "Eos: a novel member of the Ikaros gene family expressed predominantly in the developing nervous system". FEBS Letters 447 (1): 76–80. doi:10.1016/S0014-5793(99)00265-3. PMID 10218586.
• Koipally J, Renold A, Kim J, Georgopoulos K (Jun 1999). "Repression by Ikaros and Aiolos is mediated through histone deacetylase complexes". The EMBO Journal 18 (11): 3090–100. doi:10.1093/emboj/18.11.3090. PMC 1171390. PMID 10357820.
• Sun L, Goodman PA, Wood CM, Crotty ML, Sensel M, Sather H, Navara C, Nachman J, Steinherz PG, Gaynon PS, Seibel N, Vassilev A, Juran BD, Reaman GH, Uckun FM (Dec 1999). "Expression of aberrantly spliced oncogenic ikaros isoforms in childhood acute lymphoblastic leukemia". Journal of Clinical Oncology 17 (12): 3753–66. PMID 10577847.
• Hosokawa Y, Maeda Y, Ichinohasama R, Miura I, Taniwaki M, Seto M (Apr 2000). "The Ikaros gene, a central regulator of lymphoid differentiation, fuses to the BCL6 gene as a result of t(3;7)(q27;p12) translocation in a patient with diffuse large B-cell lymphoma". Blood 95 (8): 2719–21. PMID 10753856.
• Koipally J, Georgopoulos K (Jun 2000). "Ikaros interactions with CtBP reveal a repression mechanism that is independent of histone deacetylase activity". The Journal of Biological Chemistry 275 (26): 19594–602. doi:10.1074/jbc.M000254200. PMID 10766745.
• Perdomo J, Holmes M, Chong B, Crossley M (Dec 2000). "Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities". The Journal of Biological Chemistry 275 (49): 38347–54. doi:10.1074/jbc.M005457200. PMID 10978333.
• Payne KJ, Nicolas JH, Zhu JY, Barsky LW, Crooks GM (Aug 2001). "Cutting edge: predominant expression of a novel Ikaros isoform in normal human hemopoiesis". Journal of Immunology 167 (4): 1867–70. doi:10.4049/jimmunol.167.4.1867. PMID 11489963.
• Koipally J, Heller EJ, Seavitt JR, Georgopoulos K (Apr 2002). "Unconventional potentiation of gene expression by Ikaros". The Journal of Biological Chemistry 277 (15): 13007–15. doi:10.1074/jbc.M111371200. PMID 11799125.
• Dorsam G, Goetzl EJ (Apr 2002). "Vasoactive intestinal peptide receptor-1 (VPAC-1) is a novel gene target of the hemolymphopoietic transcription factor Ikaros". The Journal of Biological Chemistry 277 (16): 13488–93. doi:10.1074/jbc.M107922200. PMID 11812772.

External links

• IKZF1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)
• IKZF1 human gene location in the UCSC Genome Browser.
• IKZF1 human gene details in the UCSC Genome Browser.